Study Of The Relationship Between β-ocimene Signaling Pathway And JA/SA Signaling Pathways In Arabidopsis Thaliana

β-ocimene is one of the most commonly found monoterpenes of the volatile blends that are emitted from leaves in response to damage by herbivores or mechanical wounding. Airborne beta-ocimene emitted from plants can serve as a chemical cue for the attraction of parasitoids, also as an attractant for pollinating insects. Furthermore, β-ocimene can activate defense gene expression and enhance plant resistance fight against insect herbivores and microbial pathogens.Liu et al first repoted that β-ocimene could simutaneously induce PR1and PDF1.2gene expression in the same plant, implied that β-ocimene can disarm the antagonism between JA/SA pathways. However, the definite mechanism of β-ocimene disarm the antagonism between JA/SA pathways and the relationship between β-ocimene and JA/SA pathways are also unclear. In order to taking some acknowledge about the relationship between ocimene signaling pathway and JA/SA signaling pathway, used some Arabidophsis mutants as materials, then obtained the gene expression profiles from mutants which being treated by trans-β-ocimene through RT-PCR and Microarray in the paper.1. With a semi-quantitative RT-PCR analysis in Arabidopsis wild type, PR1(maker gene in SA pathway) and PDF1.2(maker gene in JA pathway) both induced by trans-β-ocimene; moreover, the expression also reach a peak in10μmol/L. PAL1, LOX1gene relate to synthesis and some transcription factors such as MYC2, WRKY70also with a high expressional level when induced by ocimene.2. With a semi-quantitative RT-PCR analysis in Arabidopsis mutants, we have found the palnt defense signaling pathway induced by trans-β-ocimene may is a novel signaling pathway independent on the JA/SA pathways, some transcription factors like MYC2, NPR1, WRKY70could paly important role in β-ocimene pathway.3. In Microarray:take the plant exposured to trans-β-ocimene as the test group, and the blank without any treatment as the control. According to the microarray datas, a series of expression differentially genes have been obtained, so it provides possibility and experimental base for further study of the signal molecule.