The Response Of Drought-tolerance Releated ScALDH21 And Sc288 Gene From Syntrichia Caninervis Under Desiccation

Environment stress is the main factors of affecting the growth of plants, drought is one of the major environment stress factors. Drought tolerant mosses are called poikilohydric plants, play an important role in ecological functions. Drought tolerant mosses in biological crust functions play an important role in drought mosses, outer structure, physiological and biochemical and molecular level have the response to drought, and the molecular level of the research and more focused on Gurbantunggut Desert. Study reported in the study of Syntrichia caninervsi dehydration in the process of water loss again the molecular mechanisms and external structure to adapt to appear is necessary. This paper took the S.caninervis as materials, studied the molecular mechanism in under drought stress dehydration or in the process of water loss again, this paper aims to study in S. caninervis under drought stress gene expression,this lay the foundation for further study of the drought-resistant plants. The conclusions are as follows:1 Establish a high quality extraction method of S. caninervis total RNA.2 Through the RT-PCR and electrophoresis, determine the stability strong 18srRNA genes as house-keeping genes.3 Through the semi-quantitative RT-PCR detection,the results show drought stress ScALDH21 gene expression quantity as drought stress time increased quantity highest, appear 4h express a peak, then gradually reduced. Swell to the 250bp genetic structures, and identified as aldehye dehydrogenase genes.4 Through the RT-PCR from drought stress of 270bp swell to the genetic fragments of dehydration that the gene meal with hydration in stress related, the hydration again during the amount as stress express time measured increase, increased expression appears in 1.5h, and then gradually reduce peak, express to 48h.5 Through the quantitative RT-PCR detection ScALDH2, Sc288 gene expression.Use 2-ΔΔCt method processing get red in S. caninervis to drought stress and normal environment there does exist expression differences, and semi-quantitative RT-PCR detection results are in agreement.6 The homology cloning result indicates successfully cloned an ALDH21 gene cDNA sequence (GQ245973) from S. caninervis, containning the gene ORF, and the length is 1452bp,encodes a putative polypeptide of 484 aminoacids.7 Using 5'RACE technology amplification mosses chek lap kok S. caninervis DRn gene cloning rib of the 5'missing parts, obtained the S. caninervis DRn gene full-length cDNA sequence, about the size 1000bp amplified PCR product for the product, and speculate the size and length of the agreement for 1000bp amplified PCR product size, the full length is 2289bp,...