Study On Genetic Diversity Of Rammeacris Kiangsu Of 5 Geographical Populations Based On Partial Sequences Of 16S RRNA Gene, COⅠ Gene, And COⅡ Gene

This paper studied genetic diversity genetic differentiation of Rammeacris kiangsu Tsai which collected from Taojiang of Hunan Province, Guangning of Guangdong Province, Guilin of Guangxi Province, Yongchang of Chongqing Province, and Changning of Sichuan Province, based on partial sequences of 16S rRNA gene, COⅠgene, and COⅡgene. The study results were as follows:(1) 506 base pairs of mitochondrial 16S rRNA gene were sequenced. Of these sequences, A, T, G, and C basic group average content is 32.0%,37.7%,17.6%, 12.7%. The average content of A+T is 69.7%, and the average content of G+C is 30.3%. The average percentage of A+T is much higher than that of G+C, which shows a strong A+T bias. In 506bp sequences were analysed by ClustalⅩ1.81 software, there are 504 conserved sites,2 variable sites,0 parsimony information sites, and 2 singleton sites.(2) 640 base pairs of mitochondrial COⅠgene were sequenced. Of these sequences, A, T, G, and C basic group average content are 36.8%,33.3%,13.2%, 16.7%. The average content of A+T is 70.1%, and the average content of G+C is 29.9%. The average percentage of A+T is much higher than that of G+C, which shows a strong A+T bias. In 640bp sequences were analysed by ClustalⅩ1.81 software, there are 638 conserved sites,2 variable sites,1 parsimony information sites, and 1 singleton sites.(3) 746 base pairs of mitochondrial COⅡgene were sequenced. Of these sequences, A, T, G, and C basic group average content is 39.8%,31.7%,13.2%, 15.3%. The average content of A+T is 71.5%, and the average content of G+C is 28.5%. The average percentage of A+T is much higher than that of G+C, which shows a strong A+T bias. In 746bp sequences were analysed by ClustalⅩ1.81 software, there are 742 conserved sites,4 variable sites,0 parsimony information sites, and 4 singleton sites.(4) Genetic distances shows that the distance of 16S rRNA gene from 0 to 0.004.The average distance is 0.0016. Of all 16S rRNA gene sequences, the number of the transition is 1, and its of the transversion is 1. The distance of COⅠgene from 0 to 0.0031. The average distance is 0.0016. Of all COⅠgene sequences, the number of the transition is 2, and its of the transversion is 0. The distance of COⅡgene from 0.0013 to 0.0027. The average distance is 0.0021. Of all COⅡgene sequences, the number of the transition is 4, and its of the transversion is 0. On the whole,16S rRNA gene of five geographical populations is not exist significant difference either which suggests that there is no significant variation among COⅠgene and COⅡgene of different geographical populations in Rammeacris kiangsu Tsai. It shows the genetic diversity in 5 populations of Rammeacris kiangsu is exceptionally low.(5) Using fagments of 16S rRNA gene, COⅠgene, and COⅡgene, we studied the phylogenetic relationships of Rammeacris kiangsu of five different geographical populations with NJ, ME, and UPGMA methods. The results showed that the topology of the same sequence groups by the NJ methods and the ME methods was exactly the same. And the topology of the same sequence groups by UPGMA methods is different from it by NJ methods and ME methods. Clustering relations of the topology of 16S rRNA gene, COⅠgene, and COⅡgene were constructed with same methods is not same.