| Aneuploidy is that cells or individuals possess abnormal chromosome number resulted by nondisjunction during cell division . Aneuploidy could be formed by malsegregating of sister chromotids or homologous chromosomes during mitosis or meiosis.AH kinds of factors induce aneuploidy are called aneugen. Aneuploidy in somatic cell can lead to carcinogenesis (many of carcinoma, such as carcinoma of lung, carcinoma of stomach and leukemia, relate to aneuploid production). On the other hand, aneuploidy in germ cell can make incidence of hereditary disease increase (the Down Syndrome of human is a aneuploidy). Therefore, detecting aneugen and exploring the aneuploid induction mechanism is a forcing task for human health protection, and, it can provide scientific evidences for defending genetic material of human.The project was based on the established systems of detecting aneugen of mammal somatic/germ cell by our group. The research aimed on detecting aneuploid production due to nondisjunction of specific chromosome in mammal somatic/germ cell of interphase by fluorescence in situ hybridization (FISH) . A known aneugen-colchicine (COL) and confirmed aneugen of mammal somatic cell, suspicious aneugen in mammal germ cell-Tripterygium hypoglaucum (Levl)Hutch, (THH) were subcutaneous injected male Kunming species mice,(l)According to cycle of mouse somatic cell, to kill mice 24 hours after the treatment. The bone marrow cell slides were prepared by general methods, FISH was carried out with bio-11-dUTP-labeled DNA probe specific for chromosome 8. (2) In the light of development cycle of mouse sperm, at 22 days after the treatment, sperm were collected from the cauda epididymis. FISH was applied to determine disomy sperm with biotin labeling DNA probes specific for the chromosomes 8.(3) Cultured human peripheral blood lymphocytes were treated by THH and COL in vitro and the slides were prepared by general methods after 48 hours treatment. Multi-color FISH was employed to evaluate frequencies of aneuploid with probes specific for chromosome 18 and chromosome X. The probes were detected by FISH in mouse bone marrow cell, mouse sperm and human peripheral blood lymphocyte, and we inquire into the susceptibility of chromosome specificrelate to these aneugens, in mammal somatic/germ cell and human peripheral blood lymphocyte, at last, to perfect system of detecting aneugens.The results showed that (1) In the mouse bone marrow cells, the positive materal-COL(1.5mg/kg) induce monosomic and trisomic frequencies of chromosome 8 were significantly higher than the solvent control (p<0.001); Tripterygium hypoglaucum(Levl)Hutch,(THH) induced monosomic frequencies of chromosome 8 in the three dose groups (120mg/kg,240mg/kg,480mg/kg) were also significantly higher than the solvent control (p<0.001); The trisomic frequencies of chromosome 8 were not significantly different between 120mg/kg group and solvent control (p>0.05), but the trisomic frequencies of chromosome 8 were significantly higher than the solvent control (pO.OOl) in 240mg/kg group and 480mg/kg group. (2) In the mouse sperm, the disomic frequencies of chromosome 8 were significantly higher than the solvent control (pO.OOl) in 240mg/kg group and 480mg/kg group ;The disomic frequencies of chromosome 8 were not significantly different between 120mg/kg group and solvent control (p>0.05). (3) In the human peripheral blood lymphocyte ,the aneuploid frequencies of chromosome 18 and chromosome X were not significantly different between in 0.078mg/ml group and solvent control (p>0.05), but the aneuploid frequencies of chromosome 18 and chromosome X were significantly higher than the solvent control (p<0.01) in 0.156mg/ml group and 0.312mg/ml group.From above we can conclude:The water extract of Tripterygium hypoglaucum(Levl)Hutch,(THH) induced significantly malsegregation of chromosome specific in mammal somatic/germ cell and human peripheral blood lymphocyte. The results confirmed deeply aneuploid-induction effect of Tripterygium hypoglaucum(Levl)Hutch,(THH) in m...
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