Isolation And Biodiversity Analysis On Bacteria Of Magnetite Mine Drainage

Isolation and phylogenetic analysis were carried out for bacteria from magnetite mine drainage samples collected from two Iron mines located at Shahe City of Hebei Province, China, and the biodiversity of magnetite mine samples was discussed preliminary.The isolation results showed that the bacteria quantity and species were different among different media and samples.1/5 PCA was the best for magnetite bacteria isolation. Neutrophilic bacteria were the major bacterial group of the magnetite mine drainage, improved 9K and improved 9K'media were not suitable for bacteria isolation growing in neutral environment of magnetite mine. The position of bacteria isolates on genera level and the biodiversity of magnetite mine drainage samples were discussed preliminary. The results showed that 66 strains of Jiangfeng drainage sample belonged to 50 OTUs,of which 36 OTUs showed highest sequence similarities from 98.4% to 100% with the phylum Proteobacteria,12 OTUs showed the highest sequence similarities from 98.9% to 99.9% with the phylum Actinobacteria and 2 OTUs showed the highest sequence similarities from 98.9% to 99.9% with the phylum Bacteroidetes.47 strains of Qianshan drainage sample belonged to 32 OTUs, of which 15 OTUs showed the highest sequence similarities from 90.4% to 100% with the phylum Proteobacteria,9 OTUs showed the highest sequence similarities from 99.4% to 100 % with the phylum Actinobacteria,7 OTUs showed the highest sequence similarities from 93.0% to100% with the phylum Firmicutes and 1 OTU shared the highest sequence similarities of 100% with the phylum Thermus with.The above strains were identified preliminary based on a broad range of morphological and physiological characteristics, the results confirmed the phylogenetic analysis results, and some strains could be identified to species level within the corresponding genus.52 strains including strain p1 etc. could be identified to 38 putative novel species with in 20 genera. Strains cp15 and cp26 were closest to their nearest neighbors Sneathiella chinensis CBMAI 737T with the similarity from 88.2% to 88.3%, they should be formed a new genus position in the family Sneathiellaceae.The biodiversity analysis results showed that the biological diversity of magnetite mine drainages was relatively rich. The bacterial community composition on genera was different among the two samples and the dominant populations were diverse, Asticcacaulis rose supreme in Jiangfeng drainage sample (19.70%), Brevundimonas was accounted for absolute advantages in Qianshan drainage sample (25.53%). It was presumed that the different grade and the hide deepth may be influenced the bacteria groups, which leaded to the dominant populations were different between the two samples.