Effects Of Progesterone And Interferon-τ On GnRH Signaling Dependented Pathway Gene Expression In Cultured Endometrium Cells From Bovine

Gonadotropin-releasing hormone (GnRH), the first key hormone of reproduction, is synthesized in the hypothalamus and is released in a pulsatile manner to stimulate pituitary gonadotrope-luteinizing hormone (LH) and follicle-stimulating hormone (FSH)synthesis and release.GnRH signaling pathway contain three signaling pathway:cAMP pathway, phosphoinositide pathway and MAPK pathway.The method of explant culture was used to culture Holstein cattle endometnal cell in vitro. Then the effects of progesterone and interferonτthe mRNA expressions of GnRH signaling dependented pathway gene in bovine endometrial cells were assayed by real-time PCR. The results were as follows:The method of explant culture was used to culture Holstein cattle endometrial cell.These cells were divided into two types:glandular epithelial cell and stromal cells. Most of the cells were stromal cells in these propagated cells.The expressions of GnRH signaling dependented pathway gene mRNA in bovine endometrial cells were effected by progesterone and interferonτ. Progesterone and interferonτhad inhibited the expression of Gs mRNA, the expression of Gs mRNA in control group was highly significantly higher comparing the other three treatment groups(P<0.01). Progesterone and interferonτpromoted the expression of AC mRNA, the expression of AC mRNA in the progesterone group was the highest among the flour group,and was significant higher than the control group(P<0.05).Progesterone and interferonτpromoted the expression of PKA mRNA, the expression of PKA mRNA in the progesterone and interferonτgroup was the highest among the flour group, was highly significant higher than the control group(P<0.01).Progesterone and interferonτpromoted the expression of CREB mRNA, the expression of CREB mRNA in the interferonτgroup was the highest among the flour group. The expression of CREB mRNA in the interferonτgroup and the progesterone and interferonτgroup were highly significant higher than the control group (P<0.01).Progesterone significantly promoted the expression of Gq/11 mRNA(P<0.05), interferonτsignificantly inhibited the expression of Gq/11 mRNA(P<0.05). Progesterone and interferonτpromoted the expression of PLCβmRNA, the expression of PLCβmRNA in the interferonτgroup was the highest among the flour group. Compared the control group, the expression of PLCβmRNA in the interferonτgroup was highly significant higher(P<0.01), the expression of PLCβmRNA in the progesterone group and the progesterone and interferonτgroup was significantly higher than the control group(P<0.05).Progesterone and interferonτpromoted the expression of IP3R mRNA, and interferonτsignificantly promoted the expression of IP3R mRNA in bovine endometrial cells (P<0.05).Progesterone and interferonτpromoted the expression of CaM mRNA, the expression of CaM mRNA in the interferonτgroup was the highest among the flour group,was highly significant higher than the control group(P<0.01). The expression of CaM mRNA in the progesterone group was significantly higher than the control group(P<0.05).Progesterone and interferonτpromoted the expression of MEKK mRNA, the expression of MEKK mRNA in the interferonτgroup was the highest among the flour group, was highly significantly higher the control group(P<0.01). Interferonτpromoted the expression of MKK3/6 mRNA, progesterone did not effect on the expression of MKK3/6 mRNA.Progesterone and interferonτpromoted the expression of p38MAPK mRNA, the expression of p38MAPK mRNA in the interferonτgroup was the highest among the flour group,the second was the progesterone group.Among the flour group, the expression of p38MAPK mRNA were not significant(P>0.05).