Preparation Of Porous Polymer Membrane And Its Application In Biosensors

1. A porous poly(acrylonitrile-co-acrylic acid) film based glucose biosensor constructed by electrochemical adsorptionA novel glucose biosensor was constructed by electrochemical adsorption of glucose oxidase into porous poly(acrylonitrile-co-acrylic acid) film. The obtained biosensor showed a better stability and higher sensitivity than the biosensor prepared by simple physical adsorption. Amperometric detection of glucose was evaluated by holding the modified electrode at 0.60V (vs.SCE) in order to oxidize the hydrogen peroxide generated by the enzymatic reaction. The biosensor exhibited low detection limit and good reproducibility. In addition, the optimum parameters of the biosensor elaboration as well as the effects of experimental variables such as pH, applied potential and temperature on the amperometric response of the sensor were investigated and discussed.2. Amperometric glucose biosensor based on in situ electropolymerized polyaniline/poly(acrylonitrile-co-acrylic acid) composite filmA new type of in situ electropolymerization was used for electrochemical biosensor design. The biologic film was prepared by in situ electropolymerization of aniline into microporous poly(acrylonitrile-co-acrylic acid)-coated platinum electrode in the presence of glucose oxidase. The results of EIS and SEM indicated the success immobilization for enzyme in the composite polymer film. The novel glucose biosensor exhibited good selectivity and operational stability, which showed no apparent loss of activity after 40 consecutive measurements and intermittent usage for 45 days with storage in a phosphate buffer solution(pH 6.5) at 4℃. In addition, optimization of the biosensor construction as well as effects of applied potential, pH value of solution, temperature and common interfering compounds on the amperometric response of the sensor were investigated and discussed.