| Purpose:The research had had a training that rats were force to swim in cold water namely winter swimming for twelve weeks , the aim was to presume that if winter swimming could decrease free radical production and receive the function of anti-injury of free radical by comparing the condition of serum and free radical in liver tissue and the change of immune organ coefficient in rats in different groups which swimmed at the same moving time in different temperature of water (4℃,25℃). Method:Thirty rats were divided into three groups at random, respectively Control, Warm and Cold, ten each group. Control group were bred normally, no interference. Warm group swimmed only one time each day in 25℃±5 °C water for 1 minute. Cold group swim only one time each day in 4°C water for 1 minute. After 12 weeks, being taken blood and livers of each group out,checked SOD, CAT activity and MDA' s contents, and tested LF' s contents in liver tissue to clarify that winter swimming had influence on serum and free radical in liver tissue in rats;being taken immune organ and thymus out at the same time and computed splenic and thymus organs coefficients to clarity that winter swimming had influence on immune of rats. Results:(1)General condition:Cold group had no marked hyperirritable during the trained term. After 12 weeks ,Control group and Warm group appeared aging, while Cold group were as good as before.(2)Body weight, splenic weight, thymus weight and training of each group:The weight of rats in each group increased obviously in the first four weeks of the experiment , but later gradually slowed down. When the rats were killed, the weight in cold group was significantly lower thanwarm group and control group, the mean difference is significant at the 0.01 level. (P (0.01) . splenic and thymus organs coefficients were higher than warm group and control group. The rats in each group had not been dead during experiment.(3) winter swimming could increase serum and SOD, CAT activities in liver tissue, decreased contents of serum and contents of MDA and Lf in liver tissue. Conclusions:long proper Winter swimming could increase serum and SOD, CAT activities in liver tissue, decreased contents of serum and contents of MDA and Lf and receive the function of anti-injury of free radical. |
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