Effects Of Exercise On Cerebral Glutathione And Neurotrophic Factor In Mice With Chronic Parkinson 's Disease

Objective:Based on the successful establishment of chronic mouse model of Parkinson, Research In Motion promoting effect on Parkinson’s disease in mice in behavioral science,Intervention explore movement in mouse brain glutathione, and neurotrophic factors.Method:Optional clean male C57BL/6J mice,8weeks of age40,Weight24±3g, General conditions of feeding, The mice were randomly divided into5groups, Namely:Health quiet group (A,n=8), MPTP injection group (group B, n=8), Healthy exercise group (group C, n=8), Sports+MPTP injection group (D group, n=8), MPTP injection+exercise group (E group, n=8), The experimental group of mice injected MPTP, Establish a mouse model of chronic Parkinson’s disease. Mice in each group of moderate intensity exercise group runner training (20rev/min) a day, Every30minutes, For4weeks. The various stages of mice behavioral tests and weight measurement, Water drawn from the first12hours of fasting, And mice, body weight, Behavioral testing. Finally, after a motion stimuli drawn, Detection of mice brain GSH content, GSH-PX activity, BDNF, GDNF protein levels and other indicators.Results:1. By MPTP mice by intraperitoneal injection(Dissolved in9g/L saline to a final concentration of1g/L),2times a week, Interval of three days, Every15mg/kg,4consecutive weeks successfully established chronic mouse model of Parkinson’s disease.2.Exercise group behavioral test scores compared with the quiet group significant difference (P O.01), D, E and B group compared two groups there was a significant difference(P<0.01). The D group compared with the group E, D groups were based on the behavior of sports science test data slightly better than the E group of mice (P<0.05)3.MPTP group of animals was significantly reduced brain GSH content, GSH-PX activity was significantly decreased, quiet healthy mice and mice on a quiet movement GSH content and GSH-PX activity there was significant difference (P<0.01), the D and E groups were in the comparison between the two groups of mice are shown some differences (P<0.05), and reflects a certain trend.4.Exists D expression in mice with group B,E protein in mice difference (P<0.01), showed that exercise can increase Parkinson mouse brain BDNF,GDNF protein expression, while D and E groups of mice mice brain BDNF, GDNF protein content is also relative differences (P<0.05), showed a long-term exercise habits and basic mouse brain BDNF, GDNF protein content higher than rehabilitation exercise mice.Conclusion:1. Aerobic exercise can improve behavioral manifestations of Parkinson’s disease in mice and motor function.2.PD through exercise to improve brain GSH levels in mice,enhanced GSH-PX activity, inhibit the formation of hydroxyl radicals harmful to nerve cells, played a role in the protection or restoration of nerve cells in the substantia nigra.3. Through exercise can improve PD mouse brain neurons has a significant role in protecting and promoting the restoration of BDNF, GDNF protein levels, revealing the movement for the protection and repair of dopaminergic neurons.