The Three Groups Of Chinese Herbal Compound On Rat Mesangial Cells Mmp-3/timp-1 Expression In Experimental Research

Purpose:Research the effect of three groups of chinese medicine compound prescriptions on expression of glomerular mesangial cells matrix metalloproteinases-3 (MMP-3) and tissue inhibitor of metallo proteinases-1 (TIMP-1), and probe into the effect mechanism of the effective medicine potency of delaying and preventing the glomerular sclerosis process.Material and method:1. Make three groups of chinese medicine small compound prescriptions and pharmacological blood serum of normal group by applying blood serum pharmacology method.2. Bring up rat glomerular mesangial cells by applying in vitro-culture method.3. Using AngiotensinⅡas a stimulating factor on rats mesangial cells, in order to make the cells to proliferate。4. Measure the content of fibronectin (FN) and collagen type IV in 10% FBS group and Angiotensin II group.5. Observe the expression situation of mRNA of MMP-3 and TIMP-1 among various groups by applying reverse transcription-polymerase chain reaction (RT-PCR).Results:1. Exudation of Angiotensin II group FN and collagen type IV increased significantly, and very different from that of 10% FBS group(P<0.05).2. Treatment group 1 has great promotion effect on MMP-3, and very different from that of pathology group(P<0.05), and although other groups have the current of promoting the exudation of MMP-3, it has no meaning to the statistics.3.The effect of three groups of small compound prescriptions on TIMP-1 has no meaning to the statistics. Conclusion:1. Treatment group 1 has the obvious effect of promoting the expression of MMP-3.2. Treatment group 1 will delay and prevent glomerular sclerosis by promoting the gene expression of MMP-3.3. Treatment group 2 and Treatment group 3 have the promotion effect on the gene expression of MMP-3.