| Objective:The relationship between blood estrogen level and salivary glands of female rat was studied.Methods: thirty-two female wister rats were randomly divided into four groups:control group,sham operation group,ovariectomized and injected estradiol benzoate group and ovariectomized group . Menopausal rat model was established by ovariectomy . By assayed E2, Histological examination and Cu, Zn-SOD was examined by immunochemical staining were employed for the effects of estrogen in female rat salivary .Results: Compared with control group ,sham operation group,ovariectomized and injected estradiol benzoate group ,a significant decrease in scrum estrogen level was observed in ovariectomized group(P<0.01). Observation of Histomorphology, the ovariectomized and injected estradiol benzoate group was found that there was vacuolar degeneratio in the partid acinar and the few eosinophilic changes were found in the serous submandibular gland duct epithelial and the lumen was smaller, and there was no clear change in the sublingual gland. there were more obvious changes in the ovariectomized group.In the study of Immunohistochemistry:The positive Cu, Zn-SOD in control group and sham operation group. The positive Cu, Zn-SOD in groups with estrogen infusion after ovariectomy were stronger than a group with only ovariectomy.Conclusions: Our results suggested that estrogen and Cu, Zn-SOD could suppress histologically changes in salivary glands (parotid, submandibular and sublingual ) of female rat ,that estrogen could have influence on the production... |
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