Astragalus Root Quality Research

Purpose:Based on the basic theory of TCM as the guide, combining modern science and technology, the quality of TCM astragalus comprehensive standardized research.Materials and methods:TLC method of Chinese medicine in a nucleoside astragalus astragalus the identification research, Colorimetry were used in medicine astragalus content of total ginsenosides, By orthogonal design to confirm in TCM astragalus quercetin, kaempferol formononetin, the best extraction technology, total saponins of the best extraction technology, Using HPLC measurement method, Chinese-ELSD astragalus astragalus armor in the content of mangiferin, At the same time, the method for determination of TCM with wavelength switch of astragalus quercetin, kaempferol and formononetin content, The traditional Chinese medicine and astragalus HPLC fingerprints were studied.Results:1 this method of astragalus of medicinal TLC identification, on a nucleoside astragalus spots, separating degree, Rf value and method repeatability and specificity were good.2. Of astragalus colorimetry were used in medicine, the content of total saponins of linear relationship between the good, precision, stability and repeatability and recovery are standard.3 by HPLC method for astragalus ELSD-the mangiferin contents for astragalus armour, the linear relationships, precision, stability, repeatability and recovery are standard.4 by HPLC in medicinal ingredients of astragalus benchmark, the linear relationship between the procession, precision, stability and good repeatability and recovery are standard. 5 orthogonal design to confirm the total saponins of astragalus traditional optimal extraction process conditions for:add 8 times is 70% ethanol refluxing extraction, each time, three times 2h extraction. For quercetin, kaempferol formononetin, the best extraction process conditions for:add 10 times the amount,80 percent ethanol refluxing extraction extract 1h each time, three times.6 for TCM fingerprints astragalus HPLC.Conclusion:Based on comprehensive study of standardization of astragalus medicine, astragalus membranaceus total saponins of multi-target optimum extraction technology and quality of the composition, perfecting the system of quality standards, the drug for the further research and development of astragalus reference.