Product Particles Were Scattered In The Disease For Experimental Studies Of The Human Hepatoma Cell Line Qgy-7703 Killing Effect

Purpose: Through the effects of different concentrations of disease bulk product particles on human hepatoma cell line QGY-7703 inhibited the growth rate and DNA synthesis rate of the initial reveal of disease particles scattered plot mechanism of the treatment of primary liver cancer, to further clarify the drug efficacy.Method: 1. Take exponentially proliferating human hepatoma cell line QGY-7703 cells and human lung cancer cell line SPC-A-1 cells, transfer cells, the concentration of 5×104/ml, were grown in 24-well plate (0.9ml / hole) ; the establishment of six dose groups, each dose group re-established four holes, while the positive control group and negative-based control group. Each dose of the control group re-established six holes, 37℃, 5% CO2, saturated humidity for 24 hours after the planned dose dosing 0.1ml / hole, continue to train 24 hours, supernatant concentrations of 0.25% increase trypsin 0.5ml / hole, scattered cells in 1 minute, discard trypsin, saline wash, plus normal saline 0.4ml / hole, with droppers wind and percussion suspension cells, add fetal trypan blue dye solution 0.1ml / hole, blending, stained at room temperature 5 minutes, counting living cells. 2.3.H-TdR infiltration method. Determination of DNA synthesis rate: The period of exponential growth of human hepatoma cell line QGY-7703 cells were transferred to cells in a concentration of 1×105/ml, 0.1ml / hole by adding 96-well plate, Chi 37℃, 5% CO2, culture box for 24 hours after the removal, the experimental group of patients with different doses of particles scattered plot, positive control group plus 5-FU, negative control group, normal saline, the volume, respectively 0.1ml / holes, each with six different samples in parallel, CO2 Incubators placed in foster 6h to 1uci / hole by adding 3H-TdR, continue to 1.5 hours culture, trypsin digestion 1min, cells were collected, placed dry CPM values measured by liquid scintillation.Result: 1. For human hepatoma cell line QGY-7703 half of the growth inhibition value of 462.1μg / ml, right QGY-7703 cell DNA synthesis significantly reduced compared with the control group Jie You, significant difference (P <0.01), its synthesis with the drug increase in the concentration showed a decreasing trend. San plot of disease particles on human tumor cells in vitro.2.When the dose reached 750μg / ml, its cell growth inhibition rate of more than 50%. The drug on tumor cell lines in vitro. Right QGY-7703 cells, inhibition of DNA synthesis shown in Table 2 when the drug concentration≥250μg/ml time, DNA co-inhibition rate of 50% or more, indicating accumulation of loose particles on liver disease cells in vitro.Conclusion: 1. Two indicators measured in this study results showed: the plot of disease scattered particles can be to a certain extent of anti-liver tumor cells, inhibit the liver tumor cell DNA synthesis rate, its overall efficacy is superior to positive drug control group.2.Of disease particles scattered plot with "anti-liver tumor cells and inhibit DNA synthesis rate of liver tumor cells" role, worthy of further research and development of disease particles scattered plot treatment of liver tumor is likely to be an ideal drug.