Compound Gout Particles Of Sugar-free Pharmacy And Pharmacodynamic Study

The prescription of Tong feng is according to the Hubei hospital of Tradional Chinese Medicne for treatment of experts with experience in gout research and with a new type of preparation process, by Guangcigu and Yanhusuo, JiXieTeng, etc. including six of Chinese herbs. It is beneficial for treating gout due to syndrome of heat-dampness, relieving pain. It is used in clinic to treat gout with significant effects. We based on the original clinical soup dosage, Change the forms, reform it into granules,It is not only reflect the characteristics and advantages of Chinese Medicine, but also effectively reduce dosage, and solve the diabetes patients with drugs ban sugar. In the process of research, we research Ethanol-extraction process, quality standard, the stability, pharmacodynamics and toxicology research for the preparation of provides scientific basis for clinical curative effect.In the Ethanol-extraction process we used contents of Colchicine of Guangcigu as index。Alcohol concentration, Solvent-consumption and Solvent extraction time as the main influencing factors of orthogonal method to Colchicine of Guangcigu extraction process. Results, the best condition is:alcohol concentration for 80%, alcohol consumption is three times the amount, time for six hours. In the quality standard research, we adopt thin layer chromatograph, huainiuxi and jixueteng are qualitative identified respectively. The separation of spots are good effect, negative control without interference, The method of selected strong specificity. We use HPLC to Content determination, quantitatively analyzed and methodological research. The Chromatographic analys is conditions of Colchicine is:Agilent HC ODS column (5um, C18 column-4.6 mmX250mm), mobile phase is methanol-water (45:55); 245nm detected wavelength; Temperature:25℃; velocity of flow 1.0 ml/min; The sample amount is 10μl; Separating degree not below 2.0; separating degree theory of Colchicine peak area computation not less than 3,000. Results show that the method is simple, reliable, high accuracy and good feasibility, suitable for the he prescription of Tong feng quality control.In the preliminary experimental investigation of stability, in order to verify the rationality of process conditions, we study three batch. To test the product quality (including general appearance characters, solubility, Moisture, microorganism limit inspection, TLC identification and content determination), the results show that this product is comply with the requirements.Pharmacodynamics experiment:anti-inflammatory mouse auricular swelling experiment, foot swelling-test in mice and Determination of prostaglandin PEG2 experiment. Experimental results showed that the prescription of Tong feng anti-inflammatory effect is obvious.