Salvianolic Acid B On Rat Bone Marrow Mesenchymal Stem Cell Growth And Security Impact Assessment With The Appropriate Concentration

In ischemic heart disease, including myocardial infarction, hypoxia and ischemia cause cardiac myocytes to die and dysfunction which usually lead to heart failure and cause death of people at last. Stem cells transplantation (SCT) has its strength among kinds of treatments. Bone marrow derived mesenchymal stem cells (MSCs) can be isolated from bone marrow easily and expanded in vitro without any apparent modification in phenotype or loss of pluripotent function. And cell therapy with MSCs without immunological reaction and moralo has been shown to have great promise in cardiac repair of ischemic heart disease. However, poor viability of transplanted MSCs in the infracted heart has limited the therapeutic efficacy. As we all know that Traditional Chinese Medicine (TCM) use Huoxue huayu herbs to advantage in the treatment of ischemic heart disease. The root of red-rooted salvia as a kind of Huoxue huayu herbs is used very frequently. Many studies of stem cells are about Salvianolic acid B (SalB), main element of root of red-rooted salvia. SalB is likely to preventing stem cells from apoptosis. So in this study, we choose SalB as the key medicine, isolate MSCs from bone marrow of SD rat and expand it in vitro with whole marrow method. We take pictures of every period of MSCs, and paint its growth curve. And we try to make sure the right concentration of SalB for MSCs. Through this study we find whole marrow method is efficient and know the growth rhythm of MSCs. The most important is that we have made sure the safety and right concentration of SalB which lay a foundationfor subsequent study of SalB preventing MSCs from apoptosis induced by Hypoxia/SD.