| In this paper, the author'the purpose of this research is to discuss the effect of Shudi Gouqi Dun Zhunao to the senile dementia mouse in learning and memory ability and the related blood serum, the brain organization biochemistry target influence. Selects April to May age clean level ICR healthy mouse 112, is half and half female and male, divides into 7 groups according to the body weight : blank group, model group, western medicine group, Shudi Gouqi Dun zhunao high dose group, Shudi Gouqi Dun zhunao middle dose group, Shudi Gouqi Dun zhunao low dose group and Huaishan Qizi Dun Zhunao. All groups except the blank group, uses the scopolamine (SCOP), Alcl3 and the D-gal three factor union induction, namely the 1st day starts 16:00 to fill stomach 4mL/d in the afternoon the Alcl3 peroxide solution only and injects 200mL/kg D-gal, and starts in the 5th week, noon 12:00 injects 2mL/kg the scopolamina hydrobromidum inoculation fluid, establishes the AD model. The 2nd day starts 7:30 to fill stomach 20mL/kg every day in the morning the distilled water, other each group fills the clothing commensurability the medicine food and examines each target after 6 weeks. Through the Y-maze test of learning and memory ability in mice changes in chemical colorimetric determination of serum and brain tissue malondialdehyde (MDA) content and brain tissue acetylcholinesterase (AChE) of energy, with the yellow Determination of purine oxidase superoxide dismutase (SOD) of the activity, using automatic biochemical analyzer determination of serum cholinesterase (ChE) activity and serum total cholesterol levels, and with an optical microscope hippocampus morphology in mice changes.SPSS18.0 statistical software used for statistical analysis of data and found that Compared with model group, control group and Shudi Gouqi Dun zhunao middle dose groups in body weight, learning and memory abilities, and other biochemical indicators were significant differences ( P <0.01). Western medicine group in serum ChE activity and brain AChE activity than other drugs groups, Shudi Gouqi Dun zhunao middle dose in body weight, learning and memory ability and brain tissue and serum MDA content and superoxide dismutase ( SOD).The viability so that the effect is good. In the light microscope, hippocampal CA3 area of slices in the model group were the most obvious change, Shudi Gouqi Dun zhunao middle dose group and control group did not change significantly.The following conclusions can be drawn based on the results: scopolamine, Alcl3 with the D-gal induced by three factors, the AD model mice on the ICR to build is a success. Shudi Gouqi Dun zhunao can improve the AD model of porcine brain stew of learning and memory impairment in mice. Shudi Gouqi Dun zhunao AD stew Porcine Model of pathological changes in hippocampus of mice with the improvement. Shudi Gouqi Dun zhunao SOD stew porcine brain activity can be enhanced to reduce the MDA content in the body, thereby improving the oxidation caused by oxygen free radicals due to the change to play its antioxidant activity. Shudi Gouqi Dun zhunao can inhibit AChE or stew porcine brain ChE activity, reduce the rate of hydrolysis of acetylcholine and thus increase its concentration in the patient's body and thus play a role in AD prevention and treatment. |
