The Function Of Improving The Learing-memory Ability And The Function Mechanism Of Chuanxiongqin Injection For The Treatment Of Vascular Dementia In Rats Model

Objective: To investigate the effect mechanism of Chuanxiongqin injection for the treatment of vascular dementia in animal model by means of the experimental study on the effect of the behavioral experiment , some biochemical substance in brain tissue of rats and the pathology morphological changes of brain tissue.Methods: 80 male Wistar rats were randomized into 8 groups which were categorized as follows: (1)normal group; (2)sham-operated group; (3)model group;(4) Piracetam treated group; (5) Nimodipine treated group; (6)low dose of Chuanxiongqin injection treated group; (7)middle dose of Chuanxiongqin injection treated group; (8) high dose of Chuanxiongqin injection treated group. After intraperitoneal injection of low dose of sodium nitroprusside, animal models of vascular dementia were established by clamping common carotid arteries repeatedly. After the use of medicine, some changes were observed: observe the performance record of learning and memory by the step-down avoidance test; the content of SOD,MDA,NO,Ca2+ in brain tissue were detected by means of UV spectrometer; the levels of ET,CGRP in brain tissue were detected by means of radioimmunassay(RIA); meanwhile the pathology morphological changes of brain tissue were observed under optical microscope in HE staining.Result: After treated by Chuanxiongqin injection, the learning and memory ability could be improved by the test of step-down avodiance; Chuanxiongqin injection could significantly enhance the activity of SOD,reduce the content of MDA,reduce the levels of ET and Ca2+ obviously,increase the CGRP content and lower the content of NO,compared with model group. (P<0.05 or P<0.01). The level of SOD in brain tissue of high dose of Chuanxiongqin injection treated group was higher than Piracetam treated group, Nimodipine treated group and low dose of Chuanxiongqin injection treated group(P<0.01);MDA level of high dose group was lower than Piracetam treated group, Nimodipine treated group,low and middle dose of Chuanxiongqin injection treated group(P<0.05 or P<0.01);Ca2+ level of high dose group was lower than low and middle dose of Chuanxiongqin injection treated group(P<0.05);ET level of high dose group was lower than Piracetam treated group and low dose of Chuanxiongqin injection treated group(P<0.05);CGRP level of high dose group was higher than Piracetam treated group , low and middle dose of Chuanxiongqin injection treated group(P<0.05 or P<0.01). The level of MDA in brain tissue of middle dose of Chuanxiongqin injection treated group was lower than Piracetam treated group(P<0.05).Conclusion: Chuanxiongqin injection has the function of improving the learing-memory ability in model rats, its function mechanism mainly increases the SOD content, reduces the production of MDA,and improve brain injury by strengthening the ability of brain tissue anti-oxidation;decreasing the level of NO so that toxic factors which followed were alleviated in the nerve cells and avoided the death of cell;lessen the contents of ET and Ca2+ and enhance the content of CGRP in brain tissue so that the cerebrovascular function could be amended. On the whole the effect of high dose of Chuanxiongqin injection treated vascular dementia in animal model was better than the effect of low and middle dose group. The effect of improving the level of SOD,MDA,ET,CGRP in rats treated by high dose of Chuanxiongqin injection was better than in rats treated by Piracetam.At the same time compared with Nimodipine treated group, high dose of Chuanxiongqin injection treated group could increase the SOD content and lower the level of MDA obviously.