| Ischemic cerebral vascular disease (ICVD) is well known with its high incidence, high relapse rate and high mutilation rate, high death rate. The disease not only impaired patients'health, but also brings burden to the social. Therefore, it is an important task to further definite the pathophysiological mechanism of cerebral ischemia and to develop effective drugs of treating and preventing ischemic stroke.The pathophysiological mechanism of cerebral ischemia is very complex. Previous studies have demonstrated that it related with many factors, such as energy metabolism, massive free radicals producing, vasodilator substance and vasoconstractor substance, platelet activation, etc.Steroidal Saponin TSA is the major active components of anemarrhenae .The activation of anemarrhenae has been reported in the neuroprotective manifestations on acute cerebral ischemia rats model. Regulating the blood vessel endothelium function of ischemic brain, anti-thrombopoiesis and improving blood supply are the effective mechanisms of TSA. While the researches to TSA on the cerebral blood flow and energy metabolism after cerebral ischemia haven't been reported so far. Here, in the study we were interested in the effect of TSA on the cerebral blood flow and energy metabolism after cerebral ischemia, using middle cerebral artery occlusion model during a relatively long term of 14 days. We investigated the effect of TSA on the cerebral blood flow and energy metabolism. So that we may provide experimental evidence for TSA clinical application and explore the rationality of optimal therapeutic window with Chinese medicine intervention on cerebral infarction.The main results were as follows:1. Effects of TSA on the brain blood flow after cerebral ischemia in ratsMethods:Cerebral ischemia was induced by middle cerebral artery occlusion in rats. Determinate the brain blood flow at 7 and 14 day with Ultrasound Doppler. Results:Rats receiving TSA at a dose of 15 mg/kg,30 mg/kg and 60mg/kg could inhibition the drop of brain blood flow's rate of change than vehicle treated rats.Methods:Cerebral ischemia was induced by ligaturing BCCA. Determinate the brain blood flow with LDF.Results:Rats receiving TSA at a dose of 15 mg/kg,30 mg/kg and 60mg/kg could inhibition the drop of brain blood flow's rate of change than vehicle treated rats.Conclusion:The results demonstrate that TSA can inhibition the drop of brain blood flow's rate of change.2.Protect of TSA after cerebral ischemia in ratsMethods:Cerebral ischemia was induced by middle cerebral artery occlusion in rats. Determinate the NO,NOS, LA, LDH, SOD and MDA.Results:reduce the content of NO and the vigor of TNOS. Rats receiving TSA at a dose of 30 mg/kg and 60mg/kg could reduce the vigor of INOS. Rats receiving TSA at a dose of 15 mg/kg,30 mg/kg and 60mg/kg could reduce the content of LA; rise the vigor of LDH. Rats receiving TSA at a dose of 30 mg/kg and 60mg/kg could reduce the content of MDA and enhance the vigor of SOD; TSA at a dose of 15 mg/kg and 30 mg/kg could enhance the vigor of Cu-Zn SOD.Conclusion:The results demonstrate that TSA can [rotect the brain.3. Effects of TSA on the energy metabolism related enzymes after cerebral ischemia in ratsMethods:Cerebral ischemia was induced by middle cerebral artery occlusion in rats. Determinate the Na+-K+ATP, Ca2+-Mg2+ATP, ATP,Results:Rats receiving TSA at a dose of 60mg/kg could enhance the vigor of Na+-K+ATP, Ca2+-Mg2+ATP;Conclusion:The results demonstrate that TSA can improve the energy metabolism related enzymes abstacle of the MCAO rats.4. Effects of TSA on the VIP, ET, Aâ…¡fter cerebral ischemia in ratsMethods:Cerebral ischemia was induced by middle cerebral artery occlusion in rats. Determinate the VIP, ET, AllResults:Rats receiving TSA at a dose of 15 mg/kg,30 mg/kg and 60mg/kg could rise the content of VIP;Conclusion:The results demonstrate that TSA can expand the hersen of the MCAO rats; increase brain blood flow; reduce,the damage of brain tissue.5. The effect of timosaponin-TSA on platelet aggregation of rabbits in vitro.Methods:Platelet aggregation was monitored optically in a Lumi-aggregometer. Results: With the rise of Timosaponin-TSA's concentration, the effect of inhibit rabbit platelet aggregation get strengthen. When the Timosaponin-TSA's concentration get 1.4mg/ml, it can completely inhibit rabbit platelet aggregation.Conclusion:Timosaponin-TSA could inhibit rabbit platelet aggregation induced by ADP or collagen in a dose-dependent manner in vitro.ConclusionThe results demonstrate that Timosaponin-TSA could expand the hersen; increase brain blood flow and improve the energy metabolism abstacle of the MCAO rats; and could inhibit rabbit platelet aggregation induced by ADP or collagen in a dose-dependent manner in vitro. |
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