Tong Xie Yao Fang Experimental Study On Vip, Npy Impact Of Ibs Rat Plasma And Colon Tissue

Purpose:To explore the pathogenesis of irritable bowel syndrome(IBS),and Tongxieyaofang is used to research effect of vasoactive intestinal peptide (VIP)and neuropeptide Y(NPY) in the plasma and Colon of IBS.Material and method:1.The animal we choosed is neonatal SD rats.Model rats of IBS were prepared by chronic stimulation in colon and stimulation on tails.The model was establish -ed by intrarectal stimulation of acetic acid daily in the neonatal rats between postnatal 8 and 21days for two weeks.Then,stimulation on tails with forceps clip was given for 7 days.The threshold of abdominal withdrawal reflex(AWR) was evaluated during rectal distention at postnatal 7 weeks respectively,and the changes of rectal sensitivity were identified by the abdominal electrical -activity measured at postnatal 7 weeks to prove the sucssess of the method of model.2.When the model of IBS was sucssessful,model rats of IBS were randomly divided into six groups:the low dosage of Tongxieyaofang group(GroupA),the middle dosage of Tongxieyaofang group(Group B),the high dosage of Tongxieyaofang group (Group C),the sibamin of Western medicine group(Group D),the addition of the normal control group(Group E) with the model group(GroupF).Different group received oral administration of different treatment in a different dose for one month with once in day.The respective dose of group A,B,C,D,E,F is 0.25ml/100g (2.5g/kg/d),0.5ml/100g(5g/kg/d),1ml/100g(40g/kg/d),1ml/100g(150g/kg/d),1ml/ 100g(40g/kg/d),1ml/100g(10g/kg/d).And then,We should Observe the bulgy capabi -lity of the sacculus that cause up lifting of abdomen and arching of back and the time of contract about abdomen muscle in rats.The level of Neuropeptide Y and vasoactive intestinal peptide of plama and intestinal mucosa were evaluated. The Results were analyed by statistican.Results: 1.In contrast to neonatal rats subjected to saline intrarectally and neonatal rats subjected to acetic acid intrarectally,neonatal rats subjected to acetic acid intrarectally and stimulated with forceps clip on tails showed a signif -icant decrease in the volume threshold of abdomen lifting and body arching durin -g rectal distension(P＜0.01).There was a significant increase of abdominal electrical activity responding to 0.3,0.6,and 0.9mL distention volume.2.After treatment,tongxieyaofang and Sibamin were able to make intestinal behav -ioral and electrophysiological indicators induced after sensitization returned to normal,there is no difference between the high dosage of Tongxieyaofang group and the group of Sibamin.But there is no obvious recovery in the low dosage and the middle of Tongxieyaofang group.It can't reached statistical signific -ance.The level of VIP in plasma of the model group rats increased significantly The high dosage of Tongxieyaofang group and the group of Sibamin were able to make the level of VIP descends signifycantly.And there is no difference between the high dosage of Tongxieyaofang group and the group of Sibamin.Compary to the VIP,the NPY has the opposite results.Conclusion:1.tongxieyaofang was able to make intestinal behavioral and electrophy -siological indicators induced after sensitization returned to normal.2.The level of VIP increases and NPY descends significantly,which play an important role in the pathogenesis of IBS.3.Tongxieyaofang and sibamin were able to make the level of VIP in plasma and colon descends significantly and make the level of NPY in plasma and colon increased significantly.4.The possible mechanism of Tongxieyaofang:It makes VIP in plasma and colon descends significantly.At the same time,It makes NPY in plasma and colon increases significantly.It cuts down the excitability of the dorsal horn nerve fible to raise the threshold value of visceral pain.