The Volatile Components Analysis Of Two Types Of Traditional Chinese Medicine And Two Different Reference Frame To Retain The Relationship Between The Index To Explore

As the concept of "back to nature" waving all over the world, Traditional Chinese Medicine(TCM) which is regarded as a treasure of Chinese nation,is more and more popular in the international pharmaceutical market.As the energetically advocating of development of traditional medicament and the modernization of TCM,the separation and analysis of unknown complex Traditional Chinese Medicine samples is one of the hotspots and difficulties to analytical chemistry.Plant essential oil is one kind of important effective components of TCM, whose separation and analysis is regarded to be very important.In the analysis process of volatile oil,retention indices have been widely used as a powerful means.Although a large number of studies on the retention index had been reported in the last thereabout four decades,there are so many aspects to explore in the academic study.The classification methods of natural medicines differ from the purpose and emphasis.For example,they can be classfied by sibship or by pharmic function,and the same species have many similarities in the chemical compositions and pharmacological efficacy.In the second chapter,comparative analyses of essential oils from twelve herbal medicines with pungent flavor and warm nature,like Raidix Saposhnikoviae,Fructus Xanthii,Radix Angelicae Dahuricae,Flos Magnoliae etc.,were carried out by chemometric techniques and temperature-programmed retention index method,which can be applied to the qualitative analysis of complicated system.Meanwhile,by comparing the differences and similarities of chemical components,the reason why they are classified into pungent herb medicine was explained for the first time,and some proof has been provided for the pharmacological study of pungent herb medicine.At the same time,this chapter paves the way for ChapterⅢ.In the third chapter,in order to obtain the relationship of the retention indices,a new retention index was employed as standards.Therefor,2-ketones was studied as an alternative reference series to the n-alkanes in Kovats retention indices system.In the chapter,295 compouds were measured on a HP-5MS column at a rate of 4℃·min^-1.TheⅠ^T andⅠ^K indices calculated with Eq.(2-6) show a relationgship:Ⅰ^T=1.01*Ⅰ^K+183.22 R²=0.99976.The Rhizoma Curcumae family was divided into species,meanwhile, the chemical compositions in the inter-species are quite different.Thus,a single fingerprint of the essential oils' compositions in one species seems to be more reliable and more available.In the forth chapter,Chemical compositions of the Curcuma Aromatic Salisb.Oils were investigated by gas chromatography mass spectrometry(GC-MS) combined with chemometrics method.After a comprehensive analysis and identification, totally thirty-four compounds were identified,of which Eucalyptol, Elemene,Curzerene,β-Elemenone,Germacrone,Curdione and Neocurdione are the common compounds.There was a little difference in the chemical compositions between the ten samples,but in percentages on many levels.The common model of chromatographic fingerprints established on the ten Curcuma Aromatic Salisb.Oil samples provide theoretical basis for appraisal and quality control of commercial Curcuma Aromatic Salisb.Oil with a view to achieving quality control measure on medicine Curcuma Aromatic Salisb.Oil.