Chantui Extract Extraction Process Optimization And Pharmacodynamic Study

Objective: To study the best extraction technology of Periostracum Cicadae (PC) and the anti-oxidation effect in vitro of PC; To study the antitussive effect in 95 percent ethnol extract from PC and the effect of allergic contact dermatitis (ACD). This research is to provide theoretical basis for the further development of PC in clinic application.Methods: The extraction technology of PC was optimized by U5(53) Uniform Design. Extrasomatic antioxidative activities were determined from PC by xanthine oxidase method and Electron Spin Resonance (ESR) separately.The animal model of cough was induced by ammonia water stronger and citric acid. The latency of cough and the times of coughing in 3 minutes were observed to evaluate the relieving cough effects of the extract of PC95;The animal model of ACD was induced by repeatedly challenging the ear of previously sensitized rats with dinitrofluorobenzene (DNFB). The thickness of the inflamed ear regions and associated immune cytokine in the ear tissues homogenate or in serum were measured to investigate the effect of PC95 on ACD animal model.Results: The best extraction technology from PC was refluxing method ,which was water as solvent , extraction time 3h and solid-liquid ratio 1:20,3 times ; The extract of 95% ethanol showed the greatest antioxidative activity comparied with extracts from different technology extracts. Therefore, in the animal experiment, the extract of 95% ethanol(PC95) was used as a studying object. The extract from PC95 was able to lengthen obviously latency of cough (P<0.05) and decrease the number of coughing in 3 minutes significantly (P<0.05).Besides, PC95 could decrease evidently swelling degree of ear tissue caused by pathological changes, significant differences were shown between PC95 group and model group(P<0.05). PC95 could also inhibit notably releasing of ACD allergic medium induced by DNFB and development of inflammation.Conclusion: According to experimental results above metioned ,we can conclude that the best extract technology from PC by U5(53) Uniform Design was able to guide industrial production; 95% ethanol showed the greatest antioxidative activity, better relieving cough effect , inhibiting notably releasing of ACD allergic medium induced by DNFB and development of inflammation,relieving tissue damage, this study has provided theoretical basis for the further development of PC in clinic application.