Anti-flu Spray Pharmacodynamics, And Related Mechanism Of Action Studies

Objective:to observe the effects of antivirous,antibacterious, anti-inflammation,anti-allergic,antifebrile,then,the mechanism of anti-inflammation and the toxicity of FG-PWJ by nasal administration in animals,which has a curative effect against cold-catching in clinic.Methods:several animal models were chosen to study on the effects and toxicity of FG-PWJ.Additionally,the nasal allergy model induced by TDI in guinea pig was used to study on the mechanism of anti-inflammation,Inflammatory symptoms,IgE,Nuclear Transcription Factor-kappa B,Substance P were assayed by Immunocytochemistry technology and ELISA.Results:1.The Result shows inhibitory effects against Gram-positive,Gram-negative bacterium,epiphyte,anaerobic bacillus,Pylorus helix bacillus,restraint effect against the agglutinate activity of the Chicken red blood cell By influenza virus A/Sichuan/345/97/H3N2 and A/PK/8/20/H1N1.2.FG-PWJ might rapidly reduce the high temperature of rat modeled by yeast.3.FG-PWJ has the prevention effect against Delayed-type Hypersensitivity reaction caused by DNCB on the mice model.4.FG-PWJ can obviously inhibit the ear-swelling induced by xylene in mice,the increasing of celiac capaillary permeability induced by acetic acid in mice and the edema of paw induced by carrageenan. 5.FG-PWJ can inhibit the express of NF-kappa B and Substance P. Furthermore,it can decrease IgE,alleviate the nasal symptoms on the cavy model induced by TDI.Conclusions:It is concluded that FG-PWJ have the effects of antipathogenic microorganism,antifebrile,anti-inflammatory and anti-allergic. It suggests that the mechanism of anti-inflammatory may possibally be related to decreasing the express of NF-kappaB,Substance P.