Gentiana Ingredients In Transdermal Electroporation Under

Electroporation technology is an effective transdermal drug delivery mean which has aroused great concern in the field of transdermal drug delivery system both at home and abroad.In this paper,water-soluble components of gentiana were used as a model to study its role in the electroporation transdermal under the law.Based on permeation experiments in vitro and in vivo,the influence of electrical parameters of electroporation on transdermal impact on multi-components of gentiana,the difference of in vitro and in vivo permeation experiments was investigated.We also studied the protective mechanism and safty of electroporation.We draw the conclusions as follows:(1) Fingerprint technique was firstly used for evaluating the rules of electroporation on the transdermal transport of gentiana.As two indicators,the receiving fluid gentiopicroside content and similarity were analyzed.The correlation coefficient is 0.957,which shows that the two are basically the same as the law changes.So we can use the changes of single component gentiopicroside under different electrical parameters to describe the transdermal laws of multi-components within gentiana.(2)We compared the effect of electroporation in vitro and in vivo percutaneous penetration and found that the optimal electrical parameters of the two conditions are basically the same.So we can explore the laws of transdermal through in vitro experiments. At the same time,the amount of drugs that through skin is significantly different.Penetration tests in vivo should be carried out to reduce the risk.(3) We found that the dense structure of the stratum corneum become loose structure, which can reduces the cuticle on the role of drug penetration barriers.Experimental results show that the best electrical pulse is generated by circular electrode.And for the first time we found that penetration agent of water-soluble can enhance the force of electroporation.(4) The main facters arc pulse voltage and pulse frequency,which have a threshold of adverse reactions arc as follows:pulse voltage 300 V,pulse frequency 120 pulses/min.This paper demonstrated that electroporation technique can increase the amunt of traditional Chinese medicine absorption.Electroporation technology can be widely used as long as it is good for others.