High. Pin1 Expression To Adjust The Preliminary Study Of The Relevant Genes The Impact And Pin1 Downstream Of Non-small Cell Lung Cancer Cell Line The GLC82 Growth And Metastasis

The reversible phosphorylation of proteins on serine or threonine residues preceding proline(Ser/Thr-Pro) is a major cellular signaling mechanism,which plays an important role in cell metabolism process.Pin1 can specifically binds only the phosphorylated Ser/Thr-Pro motif in certain proteins and isomerizes the phosphorylated Ser/Thr-Pro bond, which induces conformational changes in proteins to regulate protein function.We have report that Pin1 is abnormal expression in lung cancer and the overexpression of Pin1 is associated with lymphoid metastasis.Here we studied the function of Pin1 about growth and metastasis in non small lung cancer cell line in two part.1 The effects of Pin1 overexpression on the growth and metastasis of non small lung cancer cell line GLC82We transfected Pin1 plasmid into Pin1 low expression non small cell line GLC82,and selected stable Pin1 high expression clone for the study of effects of Pin1.We find that Pin1 high expression can promote cell growth and enhance the ability of clone formation by cell growth experiment and colony formation assay.The ability of adhesive and migration and invasion were enhanced induced by exogenous expression of Pin1.The wound healing assay also proves that Pin1 high expression promote cell migration.2 The regulation of downstream gene by Pin1 and the meta-analysis of MDM2 polymorphismPin1 can regulate the activity of some transcription factors,so Pin1 may regulate some gene associated metastasis indirectly.We use Realtime quantity PCR to detect the expression lever of the four metastasis associated gene CDH2,VEGF,MMP2,MMP9.The results demonstrate that CDH2 and VEGF are up regulated significantly in Pin1 high expression cell line. Pin1 can bind phosphorylated P53 and enhance the P53 stability and transcription activity,which influence the MDM2 expression lever and is associated with tumorigenesis.To further understand the MDM2 and Pin1, we access the relation of SNP309 in MDM2 promoter and lung cancer susceptibility through meta-analysis in total.Then we find there is no significantly association between SNP309 in MDM2 promoter and lung cancer susceptibility.