Adaptive Analysis Of The Origin Of Fritillaria Cirrhosa And The Harvesting And Processing Methods Based On Component Analysis

Fritillaria cirrhosa D. Don, one of main original source of BULBUS FRITILLARIAE CIRRHOSAE recorded in China Pharmacopoeia, is in danger dued to excessive excavation. Semi-cultivation is one of effective way for relieving the shortage pressure for demand and keeping sustainable utilization and development. On the other hand, as the optimal collection time and processing methods are 2 key factors for herb medicine quality, it is urgently needed to carry out best collection period and processing methods related investigation.Objective:This thesis aims to investigate the adaptability of producing area area for semi-cultivation of F. cirrhosa D. Don and optimal collection period and dessicatting methods.Methods:To analyze adaptability of producing area for Fritillaria cirrhosa D. Don. by TCMGIS-I ; Based on the appropriate producing area ananlysis, semi-wild cultivation material in Proterozoic was ananyzed to find out optimal collection time and dessicating method. An acid dye Colorimetry method was established for the determination of total alkaloids that was detected against Imperialine as an index at 414 nm; And a HPLC-ELSD method was developed by detecting Peimissine. an universal alkaloid in different origins of F. cirrhosa D. Don.Results:1. Adaptability analysis of producing area showed that best producing area (Similarity index from 90% to 100%) and sec-producing area (Similarity index from 80% to 90%) is consistent with traditional main producing area. In addition, there are a few producing area that has never been reported before.2.The content of total alkaloids and yield of drug were considered as major factors for evaluation of the optimal collective period of time, and our study display that the capsule ripened is the optimal time for semi-wild cultivation material. The content of Peimissine and total alkaloids together with apparent characteristic were considered as major factors for evaluation of the processing and desiccating method, our study show that the method of washing following by desiccation with 60℃is the best one compared to others.Conclusions:The outcome of proper producing area analysis based on TCMGIS-I technology is reasonable and reliable. TCMGIS-I provides scientific data for evaluation appropriate producing area of semi-cultivation; To collect the Fritillaria cirrahosa D. Don by the time of capsule ripened, more bioactive ingredients can be accumulated, much more yield can be obtained. Meanwhile, seed is able to be cellected for enlarging the population. The method of washing following by desiccation with 60℃is simple, rapid for desiccation with maximum effective bioactive compounds.Innovation:TCMGIS- I was introduced into our study of analyzing the producing of F. cirrahosa D. Don.Total alkaloids of F. cirrahosa D. Don at different growth phases and development stages were determined and changes of total alkaloids were tracing preliminary; Drug growth at the same ecotope and collection time were colleted and analyzed to evaluate which is the best method. In this way, differentiae between different processing methods can be embodied well.Our results give some data and technique foundation for Good Agriculture Practice for Chinese Crude Drugs (GAP), redeem some disadvantages of traditional method.