Matsutake In Polysaccharide Extraction And Purification Of The Study

This paper matsutake Fruitbody polysaccharide a systematic extraction and purification. Using ultrafiltration on matsutake Fruitbody purified polysaccharides,the concentration, pressure,temperature,pH and other factors affecting in-depth and meticulous research, optimization of operating conditions set is conducive to the development of new products.The single factor test and orthogonal test to determine a better extraction process,the water than expected for the 1:30;extraction temperature:120℃;extraction time:3 h. Extraction than twice the number in the economy,two cumulative rate of 93.10%extraction. After the extract concentration of alcohol-three times the size of the conditions for the 95% ethanol alcohol Shen 6 h.The establishment of thÄ“separation and purification of polysaccharides Tricholoma matsutake technical routes,major steps include:Ultrafiltration purified by anion and cation exchange resin from protein,grade alcohol precipitation and gel filtration chromatography.The experimental results show that:Tricholoma matsutake polysaccharide protein available from the following process:Tricholoma matsutake coarse polysaccharide solutionâ†'10,000 molecular weight retention rate ultrafiltration membraneâ†'Withholding ofâ†'A resin anion and cation exchange columnâ†'Freeze-driedâ†'Tricholoma matsutake from polysaccharide protein powder.Ions resin used to the removal of Tricholoma matsutake polysaccharide protein effects reasonably well.With weak alkaline yin and weak acid cation exchange resin series,Deionized water elution conditions are mild,part of a bleaching effect,Tandem ions with the 1st,protein removal rate of 89.7%,polysaccharide recovery reached 82.1%.The method is the exchange of large capacity,industrial enlarge easy resin can be long-term repeated use,simple process,the advantages of low production costs.Through grade alcohol can be divided into two preliminary Tricholoma matsutake polysaccharide components:ABMFZ named ABMFI and,in the rates were 48%and 46%.Through Sepharose6.Gel filtration ABMFI isolated from the two polysaccharides homogeneous components:the name ABMPâ… and ABMPâ…¡. Through Sephacry S-300 gel filtration from ABMF2 polysaccharide obtained a uniform component,named ABMPâ…¢,ABMPâ…¢ABMF2 the content of 28.6%.