The Viper Chemical Ingredients

First,we extracted Agkistrodon halys' meat using the buffer saltthen extracted with the PE,the CHCl₃-MeOH(2:1) and the MeOH.The part of PE.We were identified 3-cholesterol,hexadecyclic acid,octadecylic acid,9-octadecenic acid by GS-MS; and separate cholesterol from part PE.Using the solid phase and ODS column for the MeOH part.We separated and purified three ingredients.We identified hypoxanthine,uracil and adenosine by ¹HNMR and ¹³CMNR.Used the electrophoresis method and the double Mark method compared the protein of the Agkistrodon halys' head,the skin,the meat.Besides,established water-soluble ingredients pingerprint of the head,the skin,the meat.Three part chemical ingredients were similar,but the content was different,the ingredient in the Agkistrodon halys'meat is the most.A high performance liquid chromatography/electrospray ionization/ mass spectrometry/mass spectrometry(HPLC/ESI/MS²) method has been developed for the qualitative analysis of Agkistrodon halys.The ultraviolet(UV) chromtogram, total ion current chromatogram(TIC) and extracted ion chromatogram(EIC) of the sample and ESI/MS² spectra of the peaks in the chromatograms were obtained. Uracil,hypoxanthine,inosine and guanosine in the Agkistrodon halys were identified.Has carried on the anti-oxidation experiment to the water-soluble ingredient in Agkistrodon halys'meat,uses the DPPH free radical percentage clearance as the target,proved that water-soluble ingredient in the Agkistrodon halys' meat has a certain oxidation resistance.Has established the complete quality specification of the Agkistrodon halys.