| [Objective]: To explore the relationship between the polymorphism of -149C/T(C46359T, GenBank accession no. AL035071)and -579G/T(from exon 1B transcription start site,GenBank accession NT-028392)in DNA methyltansferases 3B (DNMT3B) promoter and the pathogenesis of gastric cancer in jiangsu area.[Methods and Results]: PCR-RFLP and DNA sequencing were used to analyze the genotypic polymorphism -149C/T and -579G/T of DNMT3B promoter in genomic DNA of blood lymphocytes from 156 patients with gastric cancer and 156 normal controls. Statistical analysis was performed using the SAS9.1.3 software package. Hardy-weingberg equilibrium assumption was performed to compare the observed and expected genotype frequencies using X2 test. For the people of the gastric cancer in jiangsu area, -149C/T genotypic frequencies of 0.6% (CC), 0.6% (CT), 98.8% ( TT) were not statistically significant (P>0.05) comparing with the genotypic frequencies of 0 % (CC), 0.6% (CT), 99.4%( TT) in controls. For the people of the gastric cancer, -579G/T genotypic frequencies of 1.3%( GG), 11.5%( GT), 87.2%( TT) were statistically significant (P=0.02) comparing with the genotypic frequencies of 4.5%(GG), 19.2%(GT), 76.3%(TT) in controls. Individual with -579TT genotype were at a significantly increased risk of gastric cancer compared with those individuals with at least one -579G allele (Adjust OR=2.11, 95% confidence interval(CI)=1.16-3.84,P=0.01).[Conclusion]: The distribution of -149C/T in DNMT3B promoter may not be used as a stratification marker to predict susceptibility of gastric cancer, at least in the population of jiangsu area. This study has demonstrated different distribution of-579G/T genotype in case and control group. The -579TT homozygous were significantly associated with the risk of lung cancer. As this is the first report of this polymorphism in gastric cancer, independent studies are needed to verify this association. |
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