.csx/nkx2.5 Gene And Ccnl2 Gene In The Rat Embryonic Cardiac Development Process Of The Temporal And Spatial Expression Study

[Objective] To investigate the change of CSX/Nkx2.5 gene mRNA expression and its protein location in the developing rat hearts.[Methods] Embryonic hearts of normal rats were obtained on the 12th, 15th, 17th and 19th day during embryonic period. Reverse transcription polymerase chain reaction (RT-PCR) was used to reveal CSX/Nkx2.5 gene mRNA expression, and Immunohistochemistry for its protein location. The results were analyzed by the statistical method of One-way ANOVA.[Results] With the development of rat hearts, the expression levels of CSX/Nkx2.5 gene mRNA were up-regulated ,CSX/Nkx2.5 protein was mainly expressed in the atria , ventricles and trabecular muscles , while it was not detected in the endocardium, the epicardium , the valvular tissues , outflow tracks and large arteries.[Conclusions] CSX/Nkx2.5 gene is up-regulated in the rat heart and specifically express in the nucleus of cardiac muscle cell. It suggests that CSX/Nkx2.5 should play an important role in the fetal heart development. [Objective] To investigate CCNL2 ( cyclin L2 ) gene mRNA location during the development of Rattus heart.[Methods] Embryonic hearts of normal rats were obtained from the 11th day to the 15th day during embryonic period. CCNL2 gene mRNA location was detected by in situ hybridization.[Results] CCNL2 gene mRNA was mainly expressed in endocardial cushion , valves , outflow tracks and large arteries, and also expressed in atria, ventricles and trabecular muscles.[Conclusion] CCNL2 gene is expressed in the cell nucleus of endocardial cushion , valves , outflow tracks , large arteries and atrial ventricular myocardium. CCNL2 gene may play a role in the heart development.