Lb.paracaseihd1.7 Immunomodulatory Ability In Laboratory Mice

Since 90% of the human body and its diseases are related to immunity, the immune system to resist foreign invasion of harmful substances has played an important role in immune regulation ability.Immune regulation ability of probiotics as a screening standards and the important function of probiotics, by a wide range of concerns. The topics discussed the immune of Lb.paracaseiHD1.7 to the impact of adjusting capacity in order to understand the role of bacteria in mice, whether the activation of host immune system, thereby enhancing the host immune defense capability, to seek more, better ability to improve the regulation of the immune beneficial bacteria naturally, research and development of new health care products provide an important basis.Papers for the trial to Lb.paracaseiHD1.7 strain, will be preparing a fresh bacilli concentration for the concentration of 2×106cfu/mL, 2×108cfu/mL, 2×1010cfu/mL.gavage to the suspension of Kunming mice , In gavage 7,14,21,28 days after the detection of immune organs, immune cells, the immune elements of dynamic change. The results are as follows:(1)Thymus index: to gavage the Lb.paracaseiHD1.7 suspension for mice found, with the gavage and the extension of the increasing concentration of bacilli, the groups thymus index increased gradually, high-dose group bacilli in the gavage After 28 days, compared with the control group were significantly different, preliminary view is that high doses of Lb.paracaseiHD1.7 can promote the development of thymus.(2)Spleen index: after 7, 14 days, high-dose group of mice spleen index increased significantly (P <0.05), while low-dose group in the control group showed no significant difference, suggesting that high doses of Lb.paracaseiHD1.7 can promote the development of the spleen. (3)Macrophage phagocytes: according to the carbon clearance test we found that, with increasing doses of gavages and time of each group gavages viable phagocyte index and correction phagocyte index increased gradually, preliminary view is that there Lb.paracaseiHD1.7 Improve the macrophage phagocytes of the trend, the difference was not significant and therefore require further validation testing(4)Neutrophil tablets of cells: after 7, 21, 28 days gavages in the high-dose Lb.paracaseiHD1.7, neutrophil tablets NBT reduction of the capacity compared with the control group were significantly different (p <0.05), which gavages 21 days the most significant difference (p <0.01), while the same time the low-, medium-dose group had no significant change. Show that high doses of Lb.paracaseiHD1.7 can promote the increase in the number of neutrophil tablets.(5)T-lymphocytes: Afterα-naphthalene acetic acid esterase tests found that after gavages 7,28 days, high-dose group of T-lymphocyte percentage of the total number of lymphocytes compared with the control group differences were significant (p <0.05) and very significant (p <0.01). Lb.paracaseiHD1.7 can increase the number of T-lymphocytes.(6)Spleen T-lymphocytes: MTT spleen detected the T-lymphocyte proliferation found gavages at different times in different doses of Lb.paracaseiHD1.7, stimulated by ConA, although with the gavages and the extension of gavages, the increase in dose, the test group of spleen T-lymphocytes compared with the control group numerical increase gradually, but no significant difference. Lb.paracaseiHD1.7 can promote the trend of T-lymphocyte proliferation, but no significant differences, the need for in-depth study in order to get precise results.(4)Spleen B cells: study found that after stimulation by LPS, the test group B lymphocyte proliferation compare with the control group, although in some increase, but the difference is not significant. Although there are Lb.paracaseiHD1.7 can promote B lymphocyte proliferation trends, but no significant difference, pending further study. (8)Serum IgG content: The results showed that 7,14,21,28 of IgG in serum compared with the control group had increased the trend, but there is no significant difference. Lb.paracaseiHD1.7 can increased serum IgG in the tendency, but need further testing to be sure the results,To sum up, Lb.paracaseiHD1.7 can promote the thymus and spleen weight of the increase, in the blood can increase the number of T cells, and the ability to swallow tablets neutrophil the number of cells. Lb.paracaseiHD1.7 can improve immunity of mouse, but the macrophages,spleen T, B lymphocyte proliferation and serum IgG in no significant impact, pending further study to determine the impact of humoral immunity.