Anti-tumor Effect And Mechanism Of The Xiaoliu Tang Discussed

Objective:The eliminate tumor soup from Barbated Skullcup Herb, Chinese Lobelia Herb, Chinese sage, Hedyotic diffusa, Radix Actinidiae, Radix Astragali, Bistortae, Rhizoma Corydalis, Solanum nigrum drugs such as composition.Has the effect of clearing away heat and toxic material, softening enclosed mass, promoting blood circulation by removing blood and dewetting, adding integrity efficacy.Assist integrity and eliminating the pathogenic factor , promoting blood circulation and detumescence , removing blood stasis and removing dampness through diuresis.Compatibility of various drugs, eliminating the pathogenic factor, Attack-and carrot, curing Qi-blood, In addition to heat and toxic phlegm and blood stasis.In this study under the guidance of the theory of Chinese medicine, using serum pharmacologic method, from the cells, the molecular level, investigate the anti-tumor mechanism of Xiaoliutang systematically.To observe Xiaoliutang with medicated serum containing the Bel-7402 liver cancer cell proliferation of, the rate of apoptosis, and the cell cycle.Research on Xiaoliutang of serum containing induced apoptosis in the Bel-7402 cells.Approach the Possibility in Xiaoliutang induced liver cancer apoptosis.To provide an experimental basis for the clinical application of the treatment of cancer.Method:Optional SD rats 30, The mince were divided into five groups randomly. There were high dose Xiaoliutang group, middle dose Xiaoliutang group, low dose Xiaoliutang group, Fluorouracil group and Blank experimental cells group. Using serum pharmacologic method, Preparation of the subjects of drug-containing serum and non-serum. The experimental group by adding the cells were drug tested serum, the vacant contrast group to join non-serum, culture respectively 24h, 48h.1 By trypaning blue staining cells were detected with the Blue rate.2 By MTT (thiazole blue) was determined by each group of inhibition rate.3 FCM detect each group cells rate of apoptosis. 4 FCM detect each group cells periodic variation.Results:1 By trypaning blue Staining display, the Blue rate of cells isn't significant change.2 By Contrasting colorsof MTT, Cell survival rate lower In the circumstances of Add drugs. With the extension of time, the relative inhibition rate of increase gradually.When Xiaoliutang of serum containing low concentrations of drugs, With the inhibition of cell relatively serum containing increasing concentration of the increase. When serum containing up to a certain concentration after, the inhibition of cell relative falls in the serum concentration ncrease. Each experiment, the inhibition of cell growth is significantly different compared with blank experimental cells group. The treatment of Fluorouracil group and middle dose Xiaoliutang group than the other.3 FCM apoptosis peak, the experimental cells begin to appear apoptosis peak before in G₁ phase. As serum concentration increasing, the apoptosis peak drops first and then down, which shows a certain dose-effect relationship. Compared with different experimental cell groups, Fluorouracil group and middle dose Xiaoliutang group have the highest apoptosis peak within the same culture time.However, there is no apoptosis peak among the blank experimental cells group. Consumers in the tumor-dose group apoptosis highest peak, while the control group of apoptosis has no peak. The same group, the percentage of apoptosis role as the drug increased the time that time, 48 hours of training significantly. Various experimental drug increases the rate of apoptosis in the group with the control group, were significantly different.4 FCM apoptosis cell cycle, The cell cycle experimental group were significantly change. Compared with the blank experimental cells group, there were significant differences. For the performance of the experimental group G₁ and S period didn't change significantly, G₂～M cells increased significantly. To fluorouracil group and middle dose Xiaoliutang group have significant changes in the cell cycle.Conclusion:1 Xiaoliutang can restrain the growth of Bel-7402 cells. 2 Xiaoliutang can induce Bel-7402 cells apoptosis.3 The mechanism on molecule that Xiaoliutang induce apoptosis of Bel-7402 cells is probably related with inhibit Bel-7402 cells cycles.4 It suggests that Xiaoliutang is a potent drug for the treatment of patients with primary liver cancer.