| This paper includes two parts:one is acne's epidemic investigation and chinese medicine syndrome differentation.The other is to discuss the mechanism of asiaticoside to the fibroblast of the rat model.Part 1:acne's epidemic investigation and chinese medicine syndrome differentation.ObjectiveThis subject is to research acne's chinese medicine syndrome differntation and the risk factors of inducing or aggravating acne, further understand China acne vulgaris epidemiological trends,provide theoretical guidance to help clinical treatment.MenthodsUsing epidemiological methods,designinguniform epidemiological survey,collecting acne patients from acne specialist out-patient clinics of the first hospital of Wuhan.The investigation of sex,age,skin type, mental state,sleep time,the relationship between women acne and menstrual and the TCM differntation for statistical analysis,finally concluded.Result1.In the 2558 cases of acne patients,women 1,748 cases,male 810 cases,women is most of the treatment patients,age of Incidence is still mainly to puberty,male is to 15 to 20-year-old age group,wemon is to less than 15-year-old age group.2.Oily skin acne patients with the largest proportion,followed by neutral skin,mixed skin,dry skin.3.In the 2558 cases of acne patients,patients with spirit intension accounted for 63.29%.4.Patients with regular insufficient sleep accounted for 21.85%,patients with occasional insufficien sleep accounted for 42.65%,patients with no insufficient sleep accounted for 35.50%.5.In the 2558 cases of acne patients,phlegm and blood stasis of the guitar is most,accounted for 37.57%;Followed by liver-qi stagnation,accounted for 8.03%,damp heat guitar 26.94%,yin deficiency of liver and kidney6.53%,wind-heat of feijing0.67%.Male acne with phlegm and blood stasis of the guitar most common,wemon acne after puberty with liver-qi stagnation most common,there is no significant difference in the puberty6.In wemon acne patients there are 1103 cases having relation with menstrual, major increasing in pre-menstrual,for56.18%,followed by increasing in menstrualand increasing after menstrual,yin deficiency of liver and kidney most closely related to menstrual,followed by liver-qi stagnation.Conclusion1.In the 2558 cases of acne patients,adolescent female patients are most common. Age of Incidence is earlier in wemon than man.2.Oily skin,spirit tension,insufficient sleep can induce or exacerbate acne.3.The most common acne TCM syndrome differentation followed by phlegm and blood stasis of the guitar,liver-qi stagnation,damp heat guitar, yin deficiency of liver and kidney,wind-heat of feijing.Male acne with phlegm and blood stasis of the guitar most common,wemon acne after puberty with liver-qi stagnation most common,there is no significant difference in the puberty.Part 2:To discuss the mechanism of asiaticoside to the fibroblast of rat modelObjectiveThe experiment is to discuss the mechanism of asiaticoside to fibroblast after inflammation from the rat model.To understanding the change of ultramicrostructure of fibroblast in the inflammation developing process through observating the light mirror and the electricity mirror,providing the theoretical basis for the asiaticoside in the clinical application.Methods1.Pre-experiment1.1 With a simple Propionibacterium acnes bacteria,not complete Freund's adjuvant,Propionibacterium acnes Bacillus+incomplete Freund's adjuvant model.After the model does not give drug intervention.After the model,observe the pathological changes in different periods,to determine the inflammatory response at its peak,fibroblasts and collagen fibers at different times of change,as well as the three models in the best animal model1.2Propionibacterium acnes culture:The freezing and drying Propionibacterium acnes dissolutes in the brain heart infusion,then layes aside several minutes under the room temperature,takes the little solution culture in 5%blood agar,tires of the oxygen environment in the middle of 35-37℃to culture for 5-7 day,washes 3 times with the physiological saline,adjusts Propionibacterium acnes concertration for 2×1010/ml spare,and through light mirror confirms that it's rod-shaped Propionibacterium acnes.1.3Animal model:Shearing fur of the rat back,approximately 1×1 centimeter size,each rat four sides;then respectively injecting cultured propionibacterium acnes,incompleteFreund'sadjuvant,propionibacterium acnes and incomplete Freund's adjuvant injected into rat subcutaneous.2Experiment2.1 Propionibacterium acnes culture:With the former2.2Batching:Dividing the experiment mouse into four groups:the asiaticoside and azithromycin group,azithromycin group,asiaticoside group,azithromycin group;blank contrast group. 2.3Intragastral:2 ml of every rat.The asiaticoside+azithromycin group: asiaticoside density is 1.5mg/ml,azithromycin density is10.42 mg/ml;The asiaticoside group:asiaticoside density is 0.75mg/ml;azithromycin group:azithromycin density is5.21 mg/ml;blank Contrast group: physiological saline.According to stipulation dosage gives the medicine.in the morning 8-10 of every day.《Experimental Zoolog》Hubei science and technology publishing house)2.4.Light microscopy specimens manufacture and observation:Excising the rat tissue on 10,20,30days.Fixed with 20%formaldehyde.According to paraffin section convention processing,dyed with HE,Observes the vaccination spot to know the structural change of fibroblast under the light mirror.2.5Electron microscope specimen manufacture and observation:On 20, 30days excisions rat tissue,firstly fixes specimen with 2.5%glutaric dialdehydes,then fixes with 1%perosmic.Hands over the ethyl alcohol dehydration,Resin embedding,half thin slice localization;Slices with LKB2V ultra thin slicer,Hitachi H2600 passes the radio mirror observation. observes the vaccination spot fibroblast organ structure changing.Results1 Pre-results:Comparing three group s of ainimal models,the changes of of the inflammatory response,the fibroblasts and collagen fibers are the most obvious in the group of Propionibacterium acnes+Incomplete Freund's adjuvant.Pathological changes in different periods show that the inflammatory response on the tenth day is the most intense;on the twentith day,fibroblast prolifered mostly,collagen Increased mostly and ordered tightly.On the thirtith day,the inflammatory response of every group is weak even disappear,but there is still the proliferation of fibroblasts and collagen fibers increased thickening,better than the twentith day's.2Formal experiment results 2.1 OM results:The collagen of the blank contrast group increasing and thicking obviously,ordered tightly,the change on the thietith day's was not more obvious than the twentith day's.The change of the collagen of the aithromycin group is slightly Improved than the blank contrast group,but compared with the group before and after the improvement was not obvious.With the asiaticoside's intervention,The change of the collagen of the asiaticoside group and the asiaticoside+azithromycin group is obviosely impoved than the blank contrast group,the change on the thietith day was more obvious than the twentith day's.2.2 Electron microscope results:The fibroblasts of the blank contrast group prolifered,vlume increased,Larger number of mitochondria, swelling,RER expanded obviousely.And that fibroblasts apoptosed,the nucleus of heterochromatin increased obviousely.Changes in the cytoplasm of the aithromycin group is better than the blank contrast group's,but the change on the thietith day was more obvious than the twentith day' s.With the asiaticoside's intervention,changes in the cytoplasm of the fibroblasts are improved obviousely than the blank contrast group's, changes fibroblast closed to normal on the thirtith day.ConclusionAsiaticoside plays a role in inhibiting the proliferation of collagen, by inhibiting fibroblasts' too active biological activity,so that the function of secretion collagen decline,reduce the possibility of lefting the fibrous nodules after the inflammatory nodules of the model of the rat... |
PDF Full Text Request