Pts Saponins On Different Restore Point After Cerebral Infarction Nogo-a Expression

ObjectiveIn rat MCAO (middle cerebral artery occlusion) model, we observed the effect of PTS on neuroethology and the protein level of Nogo-A, mRNA level of Nogo-A and NgR in MCAO rat brain tissue at different time point after operation. Using electron microscope, we observed eneurosynaptic structure at the same time points. Through these experiments, we aimed to explore the mechanism of injured neurons remodeling by PTS during cerebral ischemia recovery.MethodsCreate MCAO model: male SD rat (weight=300±20 g) were used in this study, apply Longa method cause middle cerebral artery occlusion. The neurological deficit grades of cerebral infarction were estimated by Longa method. After operation, rats were randomly divided to 3 groups: model group (treat with N.S), traditional Chinese medicine (TCM) group (treat with PTS) and Western medicine group (treat with nimodipine), according to Longa. N.S, PTS and nimodipine were intragastric administration once day and lasted for 3d, 7d and 28d. Nogo-A protein, Nogo-A mRNA, NgR mRNA and synaptic structure in cortex were observed by Wester Blot, RT-PCR and TEM (transmission electron microscopy) and the changes of neurological behaviour in rats with cerebral ischemia.Results:1. Production of middle cerebral artery occlusion in rats and result of grade of neuroethologyCompared with the normal group, the grade of neuroethology was significant drop at the model group with ischemic time extension. PTS group and nimodipine group in the same period were lower than model group at different time point. Scores of neurological behaviour in rats treated with MCAO were from 1 to3. TTC staining of the brain 5 h after operation demonstrated that tissue with normal blood perfusion stained red and ischemia tissue stained white, which indicated that the rat MCAO model was made successfully.2. Effect of PTS on expression of Nogo-A protein at different time after MCAO:The experimental result show that the expression of Nogo-A of model group significantly increased at 3d, and more significantly at 7d. PTS group and nimodipine group were lower than model group at the 3 different time point.3. Effect of PTS on expression of Nogo-A mRNA and NgR mRNA at different time after MCAO:The expression of Nogo-A mRNA and NgR mRNA of model group gradually increased with ischemic time extension. PTS group and nimodipine group were lower than model group at the 3 different time point,beiside of the early ischemic.4. Effect of PTS on expression of synaptic structure at different time after MCAO:With ischemic time extension,nerve cells were leaded to karyopycnosis, chromatin mild rally, part of the nuclear membrane unclear, mitochondrial obviously decreased, mitochondrial swelling and rupture ridge and synaptic structure had changed. PTS and nimodipine can be down-regulate the expression of Nogo-A, Nogo-A mRNA and NgR mRNA, which play a role in brain protection. Under the electron microscope showed cytoplasm rich, more often chromatin, synaptic structure clear, electron density and closely myelin.Conclusion:1. PTS can reduce the neurologic deficiency in rats after MCAO.2. PTS can be down-regulate the expression of Nogo-A at different time after MCAO.3. PTS can be down-regulate the expression of Nogo-A mRNA and NgR mRNA at different time after MCAO.4. PTS can enhance the synaptic remodeling at different time after MCAO.5. PTS can decrease the expression of Nogo-A,Nogo-A mRNA and NgR mRNA and enhance the synaptic remodeling at different time in rat brain after middle cerebral artery occlusion, which may be the one of remodeling mechanisms of PTS.