| Radix Scutellariae is dry root of Scutellaria baicalensis Georg. The beginning carries is in "Shen Nong Book on Chinese medicine". This is the clinical commonly used refrigeration traditional Chinese medicine. The application history is glorious and demand for medicinal purposes is very big. It is one of large amount varieties of traditional Chinese medicine in our country, which has the good development prospect. From the synthesis of domestic and foreign literature report in recent years, we found that the main active constituent and the trace element content existence difference of Radix Scutellariae from different Originates. Radix Scutellariae from Hebei has better function of relieving fever and less content of baicalin, compare with Radix Scutellariae from Shanxi and Shandong; The grow wild Radix Scutellariae has less content of baicalin than cultivates Radix Scutellariae, but clinical acknowledges the curative effect of former. Therefore, the baicalin's content cannot completely on behalf of Radix Scutellariae 's drug efficacy quality. In certain drug efficacy function aspect (for instance: relieving fever) , Radix Scutellariae 's other active constituent is possibly playing the key role. From this explanation,it is dubious that taking the baicalin's content as Radix Scutellariae 's quality detection standard. At present , the study of the relieving fever function mechanism research regarding Radix Scutellariae limits in the sole active constituent—baicalin and scutellarin(stem leaf extraction of Radix Scutellariae) ,and research of combined action mechanism of many kinds of active constituent is quite few., which make it difficult to manifest the whole function mechanism of relieving fever. Furthermore, the mechanism research of relieving fever also concentrates in positive control mediums in temperature center, but less in thermal property cell factors and negative control mediums in temperature center. This experiment takes the Chinese medicine pharmacy theory as foundation, and takes traditional Chinese medicine Pharmacology as main research technique, to carry out experiment in view of Radix Scutellariae 's foundation effect(alleviating heat-producing factors and disintoxicating) ,in order to compare the pharmacodynamics difference of Radix Scutellariae from different Originates(Hebei, Dalian, Shanxi), and combine with traditional Chinese medicine fingerprint atlas to find out the spectrum-effect relationship in relieving fever. Then, research the whole function mechanism of relieving fever.The experiment is divided into two parts:Part 1: The mechanism research of withdraws of Radix Scutellariae in relieving feverObjectiveFrom the angle of thermal property cell factors, positive control mediums and negative control mediums in temperature center to research the whole function mechanism of Radix Scutellariae in relieving fever. Method1 The model's establishment of dry yeast making rat feverTake male Wistar rat as experimental animal, and take aspirin and dexamethasone as positive control medicine. We divide temperature qualified rats into 7 groups stochastically, each group of 8. They are aqueous extraction of Radix Scutellariae from Dalian-which content large, medium and small dosage groups (3 groups) , normal control group, model group, aspirin and dexamethasone positive control group. One hour after intragastric administration for the first time, back hypodermic injection of 20% yeast suspl is performed in each group(SPSS for normal control group ). Take the temperature at 8 hours -fever plateau.2 Observation item and methodMaximum height of temperature(ΔT/℃):The difference between maximum of temperature and BBT.Blood serum IL-1β:At the time of fever plateau, behead rats immediately, take the trunk blood and centrifugate it for supermatant, and then carry out application of sample according to instruction booklet of radio-immunity exempts box.Hypothalamus CAMP and IL-1β: At the time of fever plateau, behead rats immediately, take out of brain, get the hypothalamus in the middle of chiasm opticum and the tuber cinereum in the ice-bath, fix them in liquid nitrogen for 20min, and preserve them in -86℃icebox. Carry out application of sample according to instruction booklet of radio-immunity exempts box.Ventral septal area AVP: At the time of fever plateau, behead rats immediately, take out of brain, get the ventral septal area in the ice-bath, fix them in liquid nitrogen for 20min, and preserve them in -86℃icebox. Carry out application of sample according to instruction booklet of radio-immunity exempts box.ResultsEach group reach the fever plateau after 8 hours. Make know by the maximum height of temperature, large dosage group has better effect than medium and small dosage groups(P<0.05), and has same effect with clinical commonly used dosage aspirin(P>0.05). Aqueous extraction of Radix Scutellariae can cut down the IL-1βand CAMP content of hypothalamus, and AVP content of ventral septal area significantly (compared with model group P<0.05 ) .There are positive correlativity between maximum height of temperature and IL-1β, CAMP content of hypothalamus, and AVP content of ventral septal area (r=0.911,0.967,0.853; P<0.05) . Dexamethasone positive control group can cut down the blood serum IL-1βcontent apparently(compared with model group P<0.05 ), while the other groups cannot(compared with model group P>0.05 ).ConclusionThe effect of relieving fever of aqueous extraction of Radix Scutellariae is dose dependent. One of the whole function mechanism of Radix Scutellariae in relieving fever may be as follows: decrease the content of IL-1βin temperature center, decrease the content of CAMP and advance the releasing of AVP.Part 2: Comparison of relieving fever function about withdraws of Radix Scutellariae from different OriginatesObjectiveComparing the pharmacodynamics difference of relieving fever function about withdraws of Radix Scutellariae from different Originates, and combined with traditional Chinese medicine fingerprint atlas to find out the spectrum-effect relationship in relieving fever.Method1 The model's establishment of dry yeast making rat feverThe methods of making model and giving medicine are mentioned above. Take aspirin as comparison medicine. We divide temperature qualified rats into 9 groups stochastically, each group of 10. They are aqueous extractions (3 groups) and alcohol extractions (3 groups) of regional Radix Scutellariae groups, normal control group, model group, aspirin positive control group. Take the temperature at 0.5, 1, 2, 4, 6, 8, 10 and 12 hours respectively, and observe the change situation of temperature.2 Observation item and methodThe mean temperature curve: Take ascending height of temperature (Δt) as Y-axis, time as X-axis. Plot average temperature change curve ,according to various measuring points mean value.Maximum height of temperature(ΔT/℃):The difference between maximum of temperature and BBT.Thermal response index(TRI12): The area between average temperature change curve and baseline.ResultsThe fever rat model by dry yeast is quite stable. We had a successful model building. All withdraws of Radix Scutellariae from different Originates appear conspicuous relieving fever effect(P<0.05). Alcohol extractions have better relieving fever effect than aqueous extractions for same originate(P<0.05). Aqueous extractions from all originates have same effect with clinical commonly used dosage aspirin(P>0.05). The aqueous extraction from Hebei has better effect than aqueous extractions from the other two(P<0.05). In a word, the aqueous extraction of Radix Scutellariae from Hebei has the best relieving fever effect.Conclusion1 This experiment make a copy of fever rat model by dry yeast successfully. This model has the following characteristic: fevering stably and long-enduring make good reproducibility; higher temperature makes apparent different; the obvious inflammation is similar to endopyretic syndrome. All of above prove that this model is fit for researching the pharmacodynamics effect and the mechanism of relieving fever.2 Comparing the pharmacodynamics difference of relieving fever function about withdraws of Radix Scutellariae from different Originates.The result is that the aqueous extraction of Radix Scutellariae from Hebei has the best relieving fever effect. And then, combine the result with traditional Chinese medicine fingerprint atlas to find out the spectrum-effect relationship in relieving fever. |
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