Attenuated The Role Of The Thistle Inducing Its Compatibility Manshuriensis Renal Toxicity And Mechanism Study

Objective:To study the method and mechanism that Xiaojiyinzi and itscompatibility of herbal ingredients reduce the nephrotoxicity of caulisaristolochiae manshuriensis by experiments in vivo and in vitro.Methods:Experiments in vivoDividing the mice into seven groups randomly, the groups were fedwith physiological saline, caulis aristolochiae manshuriensis decoction,Xiaojiyinzi decoction or caulis aristolochiae manshuriensis mixed intochief, adjuvant, assistant and courier herbs in Xiaojiyinzi respectivelydecoction. After three weeks we observed the general state of health ofthese mice, tested kidney function and kidney tissue morphology.Experiments in vitro1 We tested the content of Aristolochic AcidⅠof the group of Xiaojiyinziand the groups that we found out by the experiments in vivo. According tothe content of Aristolochic AcidⅠof the group of caulis aristolochiaemanshuriensis, we made every groups content of AAⅠare same.2 The HK-2 cell were cultured in vitro.3 We added the caulis aristolochiae manshuriensis decoction of differentdensity into HK-2 cells, the ratio of apoptotic HK-2 cells wasquantitatively analyzed, we found a density to study the mechanism.4 We added the every group decoction into HK-2 cells and observed themonce 4 hours, after 24 hours, the morphological variation characteristicsof HK-2 cells can be seen through the microscope, we took them photo.5 After culturing 24 hours, the ratio of apoptotic HK-2 cells wasquantitatively analyzed.6 After culturing 24 hours, the expression of TGFβ1 were assayed in thesupernatant from cultured HK-2 ceils by ELISA.Results: Experiments in vivo1 General state of health of these mice in the groups of Xiaojiyinzidecoction or caulis aristolochiae manshuriensis mixed into chief oradjuvant herbs in Xiaojiyinzi respectively decoction was better than thatin the group of caulis aristolochiae manshuriensis.2 The kidney function of these mice in the groups of Xiaojiyinzi decoctionor caulis aristolochiae manshuriensis mixed into chief or adjuvant herbsin Xiaojiyinzi respectively decoction was obviously lower than that inthe group of caulis aristolochiae manshuriensis (P＜0.01)3 The change of kidney tissue morphology of these mice in the groups ofXiaojiyinzi decoction or caulis aristolochiae manshuriensis mixed intochief or adjuvant herbs in Xiaojiyinzi respectively decoction is less thanthat in the group of caulis aristolochiae manshuriensis.Experiments in vitro1 The contents of Aristolochic AcidⅠof the group of caulis aristolochiaemanshuriensis, the group of Xiaojiyinzi and the groups of caulisaristolochiae manshuriensis mixed into chief or adjuvant herbs inXiaojiyinzi respectively were 0.6034mg/ml, 0.1060 mg/ml, 0.1119mg/ml,0.1600mg/ml.2 The HK-2 ceils were living well in vitro..3 The morphological characteristics and living statue of HK-2 cells werein accord with related issues.4 Apoptosis of HK-2 cell treated with the groups of the combination ofcaulis aristolochiae manshuriensis and chief or adjuvant herbs inXiaojiyinzi respectively was lower than that in the group of caulisaristolochiae manshuriensis (P＜0.05).5 The expression of TGFβ1 assayed in the supernatant from cultured HK-2cell treated with the groups of the combination of caulis aristolochiaemanshuriensis and chief or adjuvant herbs in Xiaojiyinzi respectively waslower than that in the group of caulis aristolochiae manshuriensis (P ＜0.01).Conclusion:1 Xiaojiyinzi, chief or adjuvant herbs in Xiaojiyinzi can reduce thenephrotoxicity of caulis aristolochiae manshuriensis.2 Xiaojiyinzi, chief or adjuvant herbs in Xiaojiyinzi can reduce thecontents of aristolochic acidⅠof the group of caulis aristolochiaemanshuriensis.3 Xiaojiyinzi, chief or adjuvant herbs in Xiaojiyinzi can reduce theapoptosis and TGFβ1 secretion of UK-2 cell that induced by caulisaristolochiae manshuriensis.Xiaojiyinzi, chief or adjuvantherbs inXiaojiyinzi can reduce thenephrotoxicity of caulis aristolochiae manshuriensis by the methods; ofreducing the contents of aristolochic AcidⅠof the group of caulisaristolochiae manshuriensis and reducing the apoptosis and TGFβ1secretion of HK-2 cell that induced by caulis aristolochiaemanshuriensis.