| Objective:To explore the mechanism of Qihuang decoction which prevents the focal cerebral infaration of the rats, by observing the microstructure change of rats' brain tissue and examining the content of the superoxide dismutase (SOD) and the moisture in the rats' brain tissue ,the content of the malondialdehyde(MDA) and the nitrogen monoside(NO) in the serum.Methods:Fifty Wistar rats which had been adjustably bred for one week, were randomly divided into five groups: a normal group, a model group, a treated group by Qihuang decoction with the small dosage, the medium dosage, the big dosage. There were ten rats in every group. After administrated for one week with Qihuang decoction (according to huangqi and dahuang as 2: 1), the rats in the model group and Qihuang groups were fixed on the dorsal position by intraperitoneal anesthesia with the 2% sodium pentobarbital. When excided the skin of the middle neck and the superficial fascia and separated the right stemocleidomastoid and stemohyoid, the common carotid artery and the pneumogastric nerve were shown. We can put the thread under the common carotid artery for preparing , ligate the ertermal carotid artery ,clamp the intemal carotid artery and scissor a small mouth near the crotch of the common carotid artery about 3mm. When inserting the special thread into the common carotid artery, we can loose the bulldog clamp and put the special thread to the intemal carotid artery. While we felt the resistance, we can know the special thread to the start of the middle cerebral artery, which to the crotch of the common carotid artery average 18.5±0.5mm. And then we should fasten the thread, fix the part of the special thread on the extravascular and sew the superficial fascia and the skin. When the rats came to themselves, they appeared the distinct hemipegia, leaning body and circumgyratly crawling. The rats were decollation after six hours. The MDA and the NO in serum of the rats were determined. The moisture and the SOD in the 1% homogenate of the rats' brain tissue were determined. And observe the microstructure change of rats' brain tissue and the nerve cells through the microscope.Results:1. Light microscope showed that the figure of the never cells in the normal rats' brain tissue were in focus and appeared taper, star and fusiform. There were light teniform areas around the cells, and the nucleolus were big, circle, deep-dyed and in the middle of the cells. Once in a while the acidophilia nucleolus were observed. In the model group, the edema appeared in the lamina corticalis. The figure of the never cells were out of focus. The clearance around the cell and the blood vessel were increscent distinctly. The nucleolus were incrassation and even dissolved. It appeared the necrotic areas and granulocytes. The drug groups were better than the model group after taken medicine, and related to the dosage with direct ratio. High drug group was close to the normal group. But the Qihuang decoction with the small dosage was clearly difference to the normal group.2. The moisture in the rats' brain tissue, MDA and NO in the model group were much higher than the normal group (p＜0.01). The content of SOD was lower (p＜0.01). The moisture in the rats' brain tissue, MDA and NO in the Qihuang groups faster raised ,and the SOD reduced the raised. There was significant difference between the Qihuang groups and the model group (p＜0.01). The effect related to the dosage with direct ratio in the drug groups, and the small dosage was more difference from the big and medium dosage groups (p＜0.05).Conclusion:Qihuang decoction could prevent the focal cerebral infarction and the mechanism was below:First, Qihuang decoction could protect the never cells and reduce their injury in the ischemia and anoxia.Second, Qihuang decoction could improvement the focal blood flow and the permeability of the blood-brain barrier, and reduce the cerebral edema.Third, Qihuang decoction could against the effect of the free radical. Fourth, Qihuang decoction could reduce the raised NO and prevent the continue injuring in the ischemia and anoxia. |
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