| Objective: The aim of this study was to investigate the effects of ginsenoside Rg3 and ginsenoside Rg3 combined with cisplatinon the growth of nude mice cervical cancer, and to explore the possible mechanism of ginsenoside Rg3 and ginsenoside Rg3 combined with cisplatinin cells and molecules levels. The growth of human cervical cancer Hela cell, apoptosis of tumor cells , expression of CD31and PeNt were observed with optic microscope, radio-immunity immunological histo chemistry, terminal deoxynucleotidy transferase-mediated dUTP nick end labeling(TUNEL), and immunohistochemical staining. The purpose of this study is to provide a new way of medication to eure cervical cancer in clinic.Materials and methods: We mixed Hela cells completely and inoculated on the right badk of twenty-eight female nude mice by hypodermic injection under the sterile condition. The xenograft grew up and appeared red hard cauliflower in 14 days. In the fifteenth day, 28 female nude mice were randomly divided into 4 groups to receive Saline(0.5ml ,qd), Rg3(5mg/kg) alone, Cisplatin (5mg/kg) alone and Rg3 (5 mg/kg) combined with Cisplatin (5mg/kg) respectively. Rg3 was given by gastrogavage once a day, for 5 weeks, Cisplatinwas injected introperitoneally once 4 days for 5 weeks. We weighed the mice and measured the size of xenograft before and after medication. Then counted the volume of xenograft and described the graph of xenograft's growth. The day after medication, we killed the nude mice and weighed the xenograft by elect.ron balance. Then counted the control rate of xenograft. The expressions of CD31 and PCNA were checked by histochemical staining. Light. paraffin slices were treated according to instruction of TUNEL test Kit. The cells whose nucleuses were stained by brown were apoptosis cells, And the blue were negative. 300 cells were counted in every Section. Ang then calculated the apoptosis rate of cells. Results: The xenograft of control group accreted in evidence at the end of medication. Other groups' accreted too. But compared with control group, they were smaller .The xenograft' s volume of Rg3 combined with Cisplatin group was smaller than other groups(P＜0.01) . Using ginsenoside Rg3 and ginsenoside Rg3 combined with cisplatin at the same time, weight of mice increased with the mice grown up, the same as the control group. But in the cisplatin group, weight of mice depressed (p＜0.05). The tumor weight of treated group was significantly lower than that of control group(P＜0.05). The control rate of xenograft was respectively: Rg3 was 49.5%, Cisplatin was 56%,Rg3 combined with Cisplatin group was 61.2%,The MVD value of treat groups were lower than that of the control group (P＜0.05).The MVD value of Rg3 group was significantly lower than that of Cisplatin group and control group. The expression of PCNA was highter in control group than in treat groups. Theapoptosis rate was respectively: control group was 4.12±1.09%, Cisplatin group was 10.51±1.24%,ginsenoside Rg3 group was 11.57±1.38% and Rg3 combined with Cisplatin group was 24.96±1.74%. Compared with control group,the apoptosis rate of trial groups was greater (p＜0.05).Conclusions: 1. Ginsenoside Rg3 and Rg3 combined with Cisplatin can significantly inhibit the growth of transplanted human Cervical Cancer in nude mice.2. Rg3 can obviously reduce the intratumoral NVD through inhibiting angiogenesis of malignant tumor.3. Ginsenoside Rg3 and Rg3 combined with Cisplatin can inhibit cell proliferation, induce cell apoptosis.4. Ginsenoside Rg3 can add the sensitivity of cisplatin and reduce side reaction of it. |
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