Hepatic Fibrosis Astrocytic Gfap, Of ¦Á-sma, Desmin And And Vinculin

Objective: There are two kinds of fibroblast In liver, one is Hepatic Stellate Cell, the other is fibroblast in portal area. We use the method of Immunohistochemistry to identify the active model of this two kinds of cell mass in hepatic fibrosis. Method: Establish the models with Wistar male mice: CCL4 injection and bile duct ligation induce the model of hepatic fibrosis, then use SABC method to test this two kinds of active cell. The first antibody is GFAP,α-SMA, Desmin and Vinculin. Result: After 48 hour injection of CCL4, the HSC around the central vein show strong positive with GFAP,α-SMA, Desmin and Vinculin staining. After 96 hour of bile duct ligation, the fibroblast in portal area express the positive result withα-SMA and Vinculin staining, but GFAP and Desmin staining don't find changes. Conclution:α-SMA and Vinculin staining have the same positive result in HSC and fibroblast. GFAP and Desmin staining is proved can be used to distinguish the activated HSC and the fibroblast in portal area.