The Expression Pattern Of P55, Paxillin And Vinculin Mrnas During Early Pancreatic Development

The protein encoding by p55 gene is conserved from worm to human,and it shares high homology with human protein HSPC117.HSPC117 was found to be related to cell adhesion, which binds to vinculin,a protein which can combine to paxillin,in order to decrease interactions of paxillin-FAK,and then it controls the survival and movement of cells.However, it is unknown the expression and function of p55,vinculin and paxillin in pancreas development.In this work,we cloned vinculin and paxillin gene from mouse pancreas, detected the expression change of p55 gene in the pancreas among e12.5,e13.5,e15.5,e17.5, newborn and adult by semi-quantitative PCR,and studied the expression profiles of p55, vinculin and paxillin at e10.5,e12.5,e13.5,e15.5 and e17.5 by in situ hybridization.It was showed that p55 mRNA level was not evidently changed among e12.5,e13.5,e15.5, e17.5,newborn and adult mouse pancreas by semi-quantitative PCR,but p55 mRNA was not detected in e10.5 pancreatic buds by whole-mount in situ hybridization.Analysis by in situ hybridization and immunohistochemistry showed that p55 was highly expressed in terminal cells of pancreatic epithelium branches at e12.5 and e13.5,and evenly distributed throughout the whole pancreas at e15.5,however it was not detected at e17.5.The expression pattern of paxillin in pancreatic epithelium is similar to that of p55,but it was also expressed at a lowlevel in mesenchymal of pancreas,vinculin was only expressed in pancreatic epithelium,and it was not changed greatly from e12.5 to e15.5,but decreased until e17.5.In conclusion,p55 is highly expressed in secondary transition period at the ends of pancreatic epithelium branches,and paxillin and vinculin is also expressed highly in this area. These results suggest that these genes might play a role in cell migration and adhesion in epithelium branches at pancreas development.