Dihydropyridine Derivatives The Synthesis Research And Daqingye Of Herbs In The Determination Of Three Active Ingredients

This dissertation has two parts, the first part is the study of synthesis the new dihydropyridine derivatives. The second part is the using HPLC method to determine the contents of three active components in three traditional Chinese medicine (TCM) and establish the new quality control standard.Dihydropyridine compounds are a kind of important calcium channel blockers for the treatment of cardiovascular and cerebrovascular diseases. It also used as a Coenzyme NADH model compounds for biomimetic asymmetric reduction. Nine 1, 4-dihydropyridine compounds without 2, 6-substituted were synthesized by two-step reactions. Five of which were new compounds. In the third chapter the cheap diosgenin was used as starting material to synthesize the new asymmetric 1, 4-dihydropyridines. Thus, the ring-opening, oxidation, hydrolysis reactions of diosgenin were carried out and intermediate 16-DPA was obtained. Furthermore, three novel routes were designed to synthesize the new dihydropyridine derivatives which including steroidal structure starting from 16-DPA.Folium Isatidis, Radix Isatidis and Indigo Naturalis are three commomly used traditional Chinese medicine. And the contents of their active components indigo, indirubin are generally used as quality control standard. The contents of tryptanthrin, indigo and indirubin in three traditional Chinese medicine were determined by HPLC method. The results showed that all three chemical substances are existed in Folium Isatidis and Indigo Naturalis; but only indigo and indirubin are in the Radix Isatidi' methanol extraction. In addition, the strange chromatographic behavior which found from the experiment was studied by TLC, HPLC and molecular polarity.