The Modified Xiaoyaosan Experimental Studies, Chronic Stress Behavior And Immune Function In Balb / C Mice

Objective:To provide experimental foundation for clinical application by the observation of effect of jiaweixiaoyaosan which influences ethology and immune function in BALB/c mice being at chronic Stress State.Methods:The stress model is made by putting BALB/c mice into a spicule pipe.The present experiment consisit of five groups- A:the normal comparison group,B:the stress group,C:the xiaoyaosan group,D:the meta-dose jiaweixiaoyaosan group,E:the homo-dose jiaweixiaoyaosan group.The ethology indexes in mice are measured by the open-field experiment.To observe the change of immue function of mice,the thymo-index and splen-index are measured,the content of CD4 and CD8,T cell subgroup in spleen constitution,are determined by using flow cytometry,IgG in blood serum by ELISA means.Results:Statistical analysis indicated that:1.The ethology indexes In the open-field experiment,The frequency of threading square of mice in group C,D,E is more than in group B (P＜0.05)and the time when mice stays in the central square in group C,D,E is much decurtation than in group B(P＜0.05).There appear no significant difference among the latter three group administered(P＞0.05).2.The immunology indexes The thymo-index in group C,D,E visibly raises more than in group B(P＜0.05),but the splen-index in all group is closed to each other at statistic(P＞0.05).The number of CD4 cell in group C,D,E remarkably increase more than in group B(P＜0.05)and The number of CD8 cell in group C,D,E remarkably decrease more than in group B(P＜0.05),accordingly the ratio of CD4 and CD8 in group C,D,E visibly raises more than in group B(P＜0.05).The value of IgG in group C,D,E obviously rises more than in group B(P＜0.05)and IgG in group B is best.Conclusion:Chronic psychic stress could cause disorder of ethology and immune function of organism.Administering xiaoyaosan or jiaweixiaoyaosan could remarkably rivalry the change of ethology and immune function,which is arise from chronic stress.All of Administering group could effectively regulate BABL/c mice immune function.The homo-dose jiaweixiaoyaosan group is best.