The Surfactant System Of Research, Vitamin C Antioxidant Effect Of Rutin

1. The influence of vitamin C on the location and antioxidant activity ofrutin in SDS micellesThe interaction of rutin with SDS micelles are investigated through the calculationof the bingding constant K and the distribution coefficient K_D, and the effects of rutin orvitamin C on the cmc of SDS are measured via conductivity detect and isothermaltitration calorimetry. The electrostatic repulsive force between rutin anions and SDSmicelles makes the cmcs of SDS solution decrease in some sort, while the addition ofvitamin C has no influence on the interaction between rutin and SDS micelles. Thoughthe existion of SDS micelles weakens the effect of vitamin C on rutin's cyclicvoltammetric properties, the synergistic antioxidant effect of rutin and vitamin Cenhances. During the process of forming SDS micelles, the free radical scavengercapacity of rutin is observably strengthened while that capacity doesn't change anylonger with the concentration of SDS after cmc 1.2. Combined antioxidant effect of rutin and vitamin C in Triton X-100micellesBeing affected by the ratio of rutin molecules to Triton X-100 micelles, rutinmolecules don't solubilize in micelles until the concentration of Triton X-100 is overcmc2, which leads to the changes on the absorption and emission spectra. The additionof vitamin C has no effect on the interaction between rutin and Triton X-100 micelles,but can influence the spectrum properties of rutin due to its acidity. The electrochemicaloxidation of rutin and vitamin C on glass carbon electrode is blocked by Triton X-100micelles, accordingly increases the oxidant potential but decreases the current. At the same time, the synergistic antioxidant effect of rutin and vitamin C also trails off withthe increasing concentration of Triton X-100.3. Investigations of antioxidant effect of rutin in CTAB micellesElectronic absorption spectra, fluorescence emission spectra, cyclic voltammetricand electron spin resonance measurements are applied to study the location of rutin inCTAB micelles with different microstructures and microenvironments, and to gaininformation about the antioxidant capacity of rutin in CTAB micelles. Rutin moleculescan be partly solubilized in CTAB spherical micelles through electronic attraction andhydrophobic forces, which leads to the bathochromic shift of absorption spectrum, thedecrease of the microenvironment polarity of pyrene and the hydroxyl radical scavengeractivity. However, CTAB rod-like micelles just congregate rutin molecules on theirsurfaces, which results in the hypochromic shift of absorption spectra on the base ofspectra in CTAB spherical micelles but heightens the antioxidant capacity of rutin.4. The effect of vitamin C on the hydroxyl radical scavenger capacity ofrutin in CTAB micelles and microemulsionIn aqueous solution, there is only one oxidant peak on glass carbon electrode withmixture of rutin and vitamin C, while in CTAB micelles solution, the oxidant peaks ofrutin and vitamin C can be separated well. The addition of vitamin C impairs theinteraction between rutin and CTAB micelles, which makes the solubilization ability ofrutin in CTAB micelles decreased, thus leads to increase the hydroxyl radical scavengercapacity of rutin, especially in CTAB spherical micelles. Though the formation ofmicroemulsion can increase the solubilization ability and solubilization space, thesolubilization priority of the antioxidant active part B ring no longer possesses, so the hydroxyl radical scavenger capacity increases. In CTAB/C₅H₁₁OH/H₂O system,n-pentanol partly counteracts the effects of vitamin C, and the influence of vitamin C onthe spectra, microenvironment polarity, cyclic voltammetric and hydroxyl scavengercapacity of rutin all come down.