| The analysis of druggery is one of the most important components in the whole pharmacology category. The quantity of the druggery directly affects people's life health and safety. Therefore the whole control of druggery plays an important role in the clinic and medical treatment.Nowdays, the trend of pharmaceutical analysis is to determine simply and reliably the trace medicine ingredient of complex samples. Therefore, Spectrophotometry has become one of the most essential study methods in the determination of pharmic products, due to its easy apparatus, simple procedure, highly sensitivity and selectivity.The aim of this dissertation was to explore simpler, more sensitive determination methods of medication, with using spectrophotometry as research means. Meanwhile the interaction of drugs with serum albumin or with amino acid was also examined. The major contents are described as follows:1. A Sensitive Method for trace Lead by Flow Injection Analysis with Mixed MicroemulsionIn this paper, a new flow injection system in association with mixed microemulsion for the microanalysis of lead was proposed. In the mixed microemulsion system of TritonX-100/n-heptane/n-butanol/water-polyethyleneGlycol-1000, a blue ion association complex produced from the reaction between lead, potassium iodide and crystal violet in the flow stream, and the spectrophotometric detection was performed simultaneously at 540 nm. The results showed that under the optimum conditions, the linear range and detection limit was 0.0-800.0 ng/mL (r=0.9980) and 13.2 ng/mL respectively. The sample throughput was 10/h. Foreign substrates effection was also investigated. The proposed method has been successfully applied to the determination of lead in the lepidium apetalum willd.2. Determination of Vc Using Discoloring Spectrophotometry in Flow Injection System A method for determination of vitamine C (Vc) by FIA- discoloring spectrophotometry was established. In the medium of HCl, a new FIA-discoloring spectrophotometry for the determination of Vc was proposed, based on the discoloring effect of Vc on the reaction between Fe3+and SCN- and the absorbance drop of red complex of Fe3+and SCN- was proportional to the amount of Vc. The detection wavelength was 480 nm and flow rate was 6.7 mL/min The results showed that under the optimum conditions, the linear range and detection limit was 0.0-6.4μg/mL (r=0.9995) and 0.18μg/mL, respectively. The sample throughput was 72/h. The proposed method has been successfully applied to determine Vc in the Vc tablet, Vc injection and Cimetidine tablet. The method is simple, accurate and very suitable to determine Vc in batches.3. Fluorescence Probe Enhanced spectrofluorimetry for the Determination ofGatifloxacin in Pharmaceutical Formulations and Biological Fluids A spectrofluorimetry for the determination of gatifloxacin (GFLX) was developed, based on the strong fluorescence of gatifloxacin after adding fluorescence probe yttrium in buffer solution (pH=7.0). Various factors of influencing fluorescence have been researched. Under the optimum conditions, the liner range was 4.00×10-8 to 1.00×10-6 g/mL and the detection limit was 3.36×10-9 g/mL (correlation coefficient r=0.9997), respectively. The relative standard deviation was 1.1% for eleven measurements of 5.6×10-7 g/mL gatifloxacin standard solution. The mechanism of sensitizing effect of probe was discussed. The proposed method has been successfully applied to determine real samples and the obtained results were in good agreement with the results of HPLC.4. Determination of Cd,Pb,Cu and Cr in Chinese Medicinal Herbs by ICP-AES and GFAASIn this paper, a rapid method for the determination of Cd,Pb,Cu and Cr in 25 kinds of traditional Chinese medicinal herbs was developed by inductively coupled plasma atomic emission spectrometry (ICP-AES) and graphite furnace atomic absorption spectrometry(GFAAS). The influence of matrix modifier on determination was investigated. Under the optimal conditions, the recoveries of analyte were in the range of 95.0-108.0%. The results showed the two methods were in good agreement and the two methods could be used as routine techniques to analyze for these heavy metals.5. Study of the Interaction between Epristeride and Bovine Serum Albumin by Spectroscopy and its Analytical ApplicationUnder physiological condition (pH=7.4), the interaction between epristeride (EP) and bovine serum albumin (BSA) was studied by UV absorption and fluorescence spectroscopy. It was found that the fluorescence intensity of BSA was quenched strongly by EP through a static quenching procedure. The association constants, the number of binding sites and basic thermodynamic parameters were obtained based on fluorescence quenching data. On the basis of the relationship between the change of fluorescence intensity of BSA (?F) and the concentration EP, the proposed method had been successfully applied to determine EP in real samples and the obtained results were in good agreement with the results of official method, HPLC.6. Study of the Interaction of Tropisetron Hydrochloride and L-Tryptophan by Spectrofluorimetry and its Analytical ApplicationA new method for the determination of tropisetron hydrochloride with L-Tryptophan in the medium pH 9.0 NH3-NH4Cl was studied, which based on the fluorescence quenching effect of tropisetron hydrochloride on L-Tryptophan. The fluorescence quenching mechanism and various factors of influencing fluorescence quenching were discussed. Under the optimum conditions, the liner range and detection limit was 0.03 to 12.0μg/mL and 0.04μg/mL (correlation coefficient r=0.997), respectively. The calibration curve equation was ?F=6.17+12.56 C (μg/mL). The proposed method had been successfully applied to determine tropisetron hydrochloride in real samples and the obtained results were in good agreement with the results of official method. |
PDF Full Text Request