Even Dogwood Decoction Of The Spontaneously Hypertensive Rat Vascular Remodeling Intervention

OBJECTIVE:1．The research uses electro-physiological technology to test carotid bloodpressure of SHR, in order to give the proof that Lian-yu decoction have certain effect on curing hypertension.2．The research uses microholography technology to measure and calculate thickness of middle tunica and diameter of aorta and mesentery artery cavity in SHR, in order to validate Lian-yu decoction have function on vascular remodeling of SHR.3．The research uses the immunohistochemistry method to measure SHR aorta VEGF,IGF-1,ERK-2s' depiction of protein, in order to explain the mechanarem of Lian-yu decoction'function on rebuild the blood vessel of SHR.METHODS:Pick up 24 SHR and 8 WKY that grow 13 weeks. All SHR are divided randomly in three groups: Model group, Nifedipine group and LY group, each group have 8 SHR, 4 male and 4 female; 8 WKY are the Normal group, also 4 male and 4 female. Feed model group with physiological saline 0．9%, Nifedipine group with liquor Nifedipine and LY group with Chinese medicine Lian-yu decoction. Once a day lasts for four weeks, then paralyze them with sodium perborate injection, cut the fur on neck, separate muscle and expose aorta, tie the aorta at one end, then open a gap 3mm away from the tie, plug the pipe into left ventricle, meanwhile, cut right atria to form a channel to let the injection go through. Inject another 300 ml Paraformaldehyde 4%, after 30 minutes, open their thorax and celiac cavity, collect aorta 6mm, mesentery artery 4mm respectively, and put them in 20 ml Paraformaldehyde 0．4% for 24 hours. Then dehydrated, clarified and darepose them with olefin. At last, make them 4μm aorta slice, and dye with HE. Observe the change under telescope of aorta and mesentery artery, use microholography system to measure and calculate thickness of middle tunica and diameter of vessel cavity of SHR aorta and mesentery artery, use immunohistochemistry method to measure expression of VEGF,IGF-1 ERK-2s'protein in SHR aorta.RESULTS:1．Compared with WKY group, Model group are with higher DBP and SBP, DBP have very significant difference and SBP have significant difference (P<0．01 or P<0．05) . LY group, its DBP ararees with significant difference (P<0．05) . Compared with Model group, LY group and Nifedipine group SHR aorta DBP and SBP both fall with significant difference (P<0．05) .2．Observe under telescope, WKY group, rats' aorta wall medium tunica and inner tunica are not thicken, vascular cavity are larger comparatively. Model group, rats' aorta wall become thicker, especially the medium tunica, vascular cavity are smaller than WKY, medium tunica leiosporous cell are a little dareorder. Compared with SHR, Nifedipine group and LY group rats' aorta wall inner tunica and medium tunica become thinner, vascular cavity morbid symptom relieves in some degree.3．WKY group, rats' mesentery artery medium tunica and inner tunica are not thicken, vascular cavity are larger comparatively. Model group, rats' mesentery artery becomes thicker, especially the medium tunica, vascular cavity are smaller than WKY, leiosporous cell layer are increasing and dareordered. Compared with SHR, Nifedipine group and LY group rats' mesentery artery inner tunica and medium tunica become thinner, vascular cavity morbid symptom relieves in some degree.Compared with WKY group, Model group, Nifedipine group rats' aorta wall medium tunica are thicker and diameter of vascular cavity are bigger than that of WKY group, and they have very significant difference (P<0．01 . Compared with Model group, LY group and Nifedipine group rats' aorta wall medium tunica thickness/ vascular cavity diameter both reduce in some degree, and they have very significant difference and significant difference respectively (P<0．01或P<0．05) .4．Compared with WKY group, Model group rats' VEGF average light density rarees, it hasvery significant difference (P<0．01 ) . Compared with Model group, LY group and Nifedipine group rats'VEGF average light density fall, and they have significant difference and very significant difference respectively( P<0．05或P<0．01)。 5．Compared with WKY group, Model group rats' ERK-2 average light density rarees, it has very significant difference (P<0．01) . Compared with Model group, LY group and Nifedipine group rats' ERK-2 average light density fall, and they have significant difference and very significant difference respectively( P<0．05或P<0．01 )。6．Compared with WKY group, Model group rats' Model group rats' IGF-1 light density rarees, it has very significant difference(P<0．01) . Compared with Model group, LY group and Nifedipine group rats' IGF-1 light density fall, and they have significant difference and very significant difference respectively(P<0．05或P<0．01 )。CONCLUSIONS:The Lian-yu decoction can lower blood pressure of SHR, restrain remodeling of aorta and mesentery artery vascular of SHR, the possible mechanism is that it reduce the expression of VEGF,IGF-1,ERK-2s' in spontaneous hypertension rats' vascular.