| AIM: To observe the effect of total haw flavonoids (THF) on Oxidative stress (OS)/ lipid peroxidation(LPO) and hepatocyte CYP2E1 expression in the establishment of nonalcoholic steatohepatitis(NASH) rats induced by fat-rich diet;to explore the possible mechanism of THF in preventing and treating NASH.METHODS: 50 male Sprague- Dawley (SD) rats were randomly divided into 5 groups (n=10 per group): the nomal group, the model group, the Essentiale N group, the large dose of THF group, and the small dose of THF group.The rats in nomal group were fed with normal diet, the rats in other four groups were fed with fat-rich diet. The rats in the Essentiale N group, the large and the small dose of THF group were respectively perfusated with 195.4mg / (kg · d) the Essentiale N suspl, 250mg/ (kg ·d) THF, 125mg/ (kg·d) THF; while the rats in the nomal and the model groups with distilled water of same volume ,once daily . At the end of the 12 weeks, all the rats were put to death. The liver pathological change was evaluated under light microscope after HE staining. Blood sample were collected for the contents detection of serum triglyceride (TG), cholesterol (CHOL), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), aspartate aminotransferase (AST ) , alanine aminotransferase(ALT), and liver tissues were obtained for the contents detection of hepatic TG , total cholesterol (TC), malondialdehyde (MDA), superroxide dismutase (SOD) and glutathione peroxidase (GSH-PX), hepatocyte CYP2E1 expression was detected with immunohischemistry. RESULTS: the NASH rat model was established sussessfully with 12 weeks'... |
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