Investigate Of Coptis Evodia Drug Compatibility Mechanism Based On The Small Intestine Special Transporter

Objective: Inthisstudy,toresearchtheprinciple ofmatch-pairofhuanglian-wuzhuyu,invitrointestinalabsorptiontests(evertedgutsacs;non-evertedgutsacs)andinsituexperimentalmodel(insituintestineperfusion)hadbeenapplied.Theobjectiveofthisstudywastoinvestigatethemechanismsof match-pairofhuanglian-wuzhuyu.Bythisstudy,aminnowhadbeen thrown outtocatchawhale——toenlightenspecialist oftraditionalChinesemedicine.Partone:Literature ReviewThe new developments of the theory in traditional Chinese medicine matchs aswell as the survey on intestinal absorption and intestinal absorption methods ofrelatingtothisresearchfieldhavebeensummarized,and theeffectsof P-glycoproteinanditsinhibitorson thetransportationofdrugshavebeensurveyed.Parttwo:ExperimentStudies1 The study of the absorption characteristics of berberine,palmatine, coptisine inhuanglianherbThe intestinal absorption of huanglian alkaloid was researched at differentconcentrations, different transport directions and in the presence of P-glycoproteininhibitor. Experimental results could show that the absorption rate trend saturation inconcentration 35~70mg/ml. The amount of huanglain alkaloid transport from serosalside to mucosal side wasmuch more than that from mucosal side to serosal side. Theamountof huanglain alkaloidtransport of the group in the presence of P-glycoproteininhibitor was much more than the control group. The three experiment results showthat the absorption characteristics of palmatine and coptisine are similar to berberine.The conclusion that palmatine and coptisine could be regarded as substrates of P-gpwasdeduced.2 Theeffectsof wuzhuyuon thetransportationofberberine,palmatine,coptisine inhuanglianherbFirstly, the concentrations of berberine,palmatine, coptisine in the test solution ofseveral rate huanglian-wuzhuyu (6:0,6:1,6:2,6:3,6:6) were determined. Experimentalresultsshowedthattheconcentrationsof berberine,palmatine,coptisinedeclinedwiththe increasing of wuzhuyu herb. Secondly, The non-everted gut sacs were used tostudytheeffectsofwuzhuyuonthetransportationof berberine,palmatine, coptisineinhuanglianherb. After matching with wuzhuyuas some ration, the intestinal absorptionofberberine,palmatine,coptisineincreasedcomparingwith huanglianalone,andtherewas an optimal ration among match-pair of huanglian- wuzhuyu. Tirdly, the model ofintestine perfusion in situ was used to study the effects of wuzhuyu on the absorptionofberberine,palmatine, coptisine in huanglian herb. In this experiment, the ration of6:1, showed that the absorption time of berberine,palmatine and coptisine wasprolonged.Therewasthesecondabsorptionpeakinthegroupofrate6?1.3 The modulationofwuzhuyuherbonintestinalP-gpfunction invitroThe non-everted gut sacs were used to study the effects of wuzhuyu on thetransportation of verapamil hydrochloride (a subtrate of P-gp). The experiment resultwas that the ration of verapamil hydrochloride and wuzhuyu in 12:5 can increase the transportation ofverapamil hydrochloride. Conclusion is thatwuzhuyu can inhibit thefunction of P-gp in small intestine, there by increasing intestinal absorption andbioavailabilityofberberine,palmatine, coptisineinhuanglian herb.Partthree:AnalysisandDiscussionThe results of the experiments were summarized, analyzed and discussed in thispart.