| Spinal Cord Injury(SCI) is one of the main loss disease being to threaten human being life , whose morbidity , disability rate , death rate are all very high, and the basic and clinical research in every country to every year have thrown into a great quantity of energy equally, whose mechanism is still not understanding , and is still the exploration stage now. At present there is no the special efficiency method in the clinic treatment of Spinal Cord Injury. The acupuncture is one of the effective methods that cures Spinal Cord Injury , having played a very important role with it`s peculiarity.The research selects and uses improvement Allen collide with the loss rat model , aims at applying the proper frequency parameter , and discussing the molecular mechanism the electricity needle improves the spinal cord injury model rat that spinal cord damaged regenerates.Firstly, we adopt motion to count score combinedly to observe rat behavior learns , and discuss nerve chemistry mechanism on the model that the electricity needle produces possible curative effect. Secondly, we give a test to NO losing organization .Finally fetch loss cross section spinal cord to organize the analysis carrying out proteide study of group , aim at finding out relevance difference egg white , and discuss the mechanism electricity needle affects the model proteide study of group. Main results are sketched now as follows:1. Behavior learns to improve aspect: We set about the mechanism studying the electricity needle cures the acute spinal cord injury model rat's. By the fact that skill last 2 hs and skill last 6 hs are on the march to the rat, behavior learns to grade. Before discovering the electricity needle group and the medicine group skill, skill queen contrast has no difference; The electricity needle forms and the skill queen contrast has equally in case that the operation group , pathological mechanism contrast group skill front, notable difference; Medicine forms and the skill queen contrast has equally in case that the operation group , pathological mechanism contrast group skill front, notable difference .2. Loss part organizes NO contents: After skill last 6 hs put an animal to death, the principle taking out loss Duan spinal cord , making use of NO and 2 second oxygen 6.9 two amidocyanogen forks Ding reaction to generate cherry-red's determines NO thickness. Discover the electricity needle group there being no difference compared with medicine group; The electricity needle group and blank space group contrast have notable difference; The electricity needle group and fake operation group contrast have notable difference; The electricity needle group and pathological mechanism collation group contrast have notable difference; The medicine group and blank space group contrast have notable difference; The medicine group and fake operation group contrast have notable difference; The medicine group and pathological mechanism contrast set of contrast having notable difference; Contrast a group and fake operation of pathological mechanism forms , the blank space group contrast has equally notable difference. The blank space group and fake operation group contrast have no difference.Point carrying out two-way gel electrophoresis on spinal cord entire the traverse loss front and back rat spinal cord entire proteide , drawing support from the Mascot software finding out difference out of expressing proteide burns. In-line ionization mass spectra applying base material assisting laser to desorb, the proteide expressing carries out an appraisal on difference, proteide burns identifying out 6 kinds successfully: Hypothetical LEF3-IAP2,Ubiquitin carboxyl-terminal hydrolase isozyme L1,Balbiani ring protein 3 precursor,Hypothetical ORF F-93,Keratin 16,Vesicle-fusing ATPase.Proteide deals with process such as breeding , withering to die , should excite reaction , the loss being to expound central nervous system further and the basis restoring mechanism having provided theory of nerve cell part difference. |
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