Preparation And Analytical Method Of The Fur Seal Skin Gelatin Plasma Substitutes

Plasma substitutes, such as gelatin, have been used gradually in the clinical application recently. This paper prepared two kinds of gelatin hydrolysate from the seal skin, and studied and analyzed those gelatin hydrolysates.First, we prepared two kinds of gelatin hydrolysate with molecular weight distribution separately adopting the method of alkaline hydrolysis and the method of hot-pressing. During the process, we determinded the molecular weight with SDS polyacrylamide gel electrophoresis (SDS-PAGE) ,controled the hydrolysis process with the high performance gel filtration chromatography(HPGFC), and optimized the process of defat and decoloration by the use of orthogonal test. Roughing filtration, fine filtration and ultrafiltration were applied to purify the crude gelatin hydrolysate, then vacuum freeze-drying was applied to make gelatin hydrolysate become power, which can be diluted to become a kind of gelatin plasma substitute.Second, the antishock effects of gelatin plasma substitute were studied through the comparison experiments with SUCCINYLATED GELATIN INJECTIM separately. The result of the initial experiment shows that gelatin plasma substitute has the certain antishock effects. At the same time, to evaluate the safety of the gelatin plasma substitute, a pharmacological study was made by the hemolytic test, the venous stimulation test, the allergy test and the acute toxicity test. The results showed that the injection with these gelatin plasma substitutes is safe under the experimental condition.At last, a method to analyse gelatin plasma substitute from the seal skin was established based on experiments. To study the component and content of amino acids, these gelatin plasma substitute were analyzed by High Press Liquor Chromatography (HPLC) coupled with ultraviolet spectroscopy. Their molecular weight and molecular weight distribution were determined by high performance gel permeation chromatography (HPGPC)。 The protein contents of gelatin plasma substitute were determined by BCA protein content assay.