| OBJECTIVE: To investigate the effects of preventing progress of glomerulosclerosis by the Huayu Xingshui recipe in rat with 5/6 nephrectomy.METHODS: The rats were randomly assigned to Huayu Xingshui recipe treatment group, control group and normal group. Hemoglobin, serum creatinine (Scr), blood urea nitrogen (BUN) and 24h urinary protein were me sured at 4th and 8th week after the nephrectomy. Renal pathology changes were evaluated at the same time. The renal tissues were stained by immunohistochemical and Masson methods. Then integra optical densit(IOD) of TGF-β1, FN, LNα -1, Coll III, Coll IV and Collagen fibrin of the glomerular were semi-quantified by image analysis system.RESULTS: In comparison with the control group, Scr , BUN and the 24h urinary protein were decreased in Huayu Xingshui recipe group(P<0.05). But the hemoglobin can not be prevented decreasing significantly (P>0.05). The immunohistochemical result showed that TGF- β1, FN, LN α -1, Coll III,Coll IV and Collagen fibrin of the glomerular were significantly decreasedin Huayu Xingshui recipe group (P<0.05—0.01) .CONCLUSION: After the treatment of Huayu Xingshui recipe, the renal function were protected leastways partly by the participant of decreasing 24h urinary protein and preventing glomerulosclerosis. |
