Cadmium-induced Apoptosis And Bcl-2 Anti-apoptotic Mechanism

Cadmium can induce apoptosis in many kinds of cells. Studying the apoptosis induced by cadmium is necessary for us to cure lots of diseases caused by cadmium. And it is also very important for the molecular toxicological study of cadmium. This paper studied some aspects of the mechanism of apoptosis induced by cadmium:1 Apoptosis caused by DNA damage induced by cadmium in HEK293Under the condition of 37℃ and 5% CO₂, HEK293 cells was incubated in DMEM medium with different concentrations of cadmium and N-acetyl-L-cysteine plus cadmium for 6-8 hours, then the apoptosis was detected by using acridine orange / ethidium bromide double fluorescent dye staining, and the DNA damage in cells was detected by DNA ladder. The results showed that apoptosis and growth inhibition in HEK293 cells was induced by cadmium. Cadmium cause apoptosis in HEK293 cells in a dose-dependent manner and different degree of DNA ladder was detected. The results also showed that N-acetyl-L-cysteine can increase the rate of apoptosis in HEK293 cells induced by cadmium. It is suggested that cadmium might cause apoptosis with DNA damage in HEK293 cells.2 Mitochondria pathway of apoptosis induced by cadmium in HEK293 cellsFluorescent dye Rhodamine123 was used to get the time course of mitochondria membrane potential. The variation of reactive oxygen species and variation of mitochondrial membrane potential production were detected by fluorescent indicator DCFH-DA and Rhodamine123, respectively. The results showed that cadmium could induce HEK293 apoptosis by changing mitochondria membrane potential rhythmic variation and decreasing it.3 Apoptosis induced by cadmium in HEK293 cells and the anti-apoptotic function of Bcl-2.Forming Bcl - 2 overexpression HEK293 cells by transfecting pcDNA3 / Bcl - 2 .